Molecular cloning, genomic organization, chromosomal mapping and subcellular localization of mouse PAP7: a PBR and PKA-RIalpha associated protein.
Molecular cloning, genomic organization, chromosomal mapping and subcellular localization of mouse PAP7: a PBR and PKA-RIalpha associated protein.
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小鼠 PAP7 的分子克隆、基因组组织、染色体作图和亚细胞定位：PBR 和 PKA-RIalpha 相关蛋白。
DOI：
10.1016/s0378-1119(03)00453-0
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发表时间：
2003
期刊：
影响因子：
3.5
通讯作者：
Papadopoulos,Vassilios
中科院分区：
文献类型：
--
作者：
Liu,Jun;Cavalli,LucianeR;Haddad,BassemR;Papadopoulos,Vassilios
外周型苯二氮卓受体（PBR）和cAMP依赖性蛋白激酶A（PKA）调节亚基RIα（PKA-RIα）相互作用的小鼠蛋白质PBR和PKA相关蛋白7（PAP 7）被鉴定并显示参与睾丸Leydig细胞中睾酮诱导的类固醇生物合成。本研究通过5′端快速扩增法对小鼠PAP 7 cDNA进行了扩增，重组得到了一个3432 bp的序列，编码525个氨基酸，分子量约为60 kDa。小鼠和人PAP 7共享85%的氨基酸同一性，并且含有保守的酰基-CoA结合蛋白/地西泮结合抑制剂（ACBP/DBI）基序。ACBP/DBI已被鉴定为能够在所有类固醇生成细胞中刺激线粒体类固醇形成的内源性PBR配体。通过筛选BAC克隆、聚合酶链反应和检索小鼠基因组数据库，克隆并组装了小鼠PAP 7全长基因。该基因长约29 kb，包括8个外显子和7个内含子。虽然它比人类PAP 7基因短，但所有外显子在小鼠和人类之间都是保守的。通过荧光原位杂交将小鼠PAP 7基因定位于染色体1H 3 -5，这与将PAP 7 cDNA序列定位于1H 4区域的小鼠基因组数据库的计算机检索一致。免疫荧光共聚焦显微镜表明，PAP 7主要定位于小鼠肿瘤Leydig细胞中的trans-Golgi器和线粒体中，与其提出的将PKA同工酶靶向富含PBR的细胞器（即线粒体）的功能一致，其中特定蛋白底物的磷酸化介导了类固醇诱导的类固醇形成。
A mouse protein that interacts with the peripheral-type benzodiazepine receptor (PBR) and the cAMP-dependent protein kinase A (PKA) regulatory subunit RIα (PKA-RIα), named PBR and PKA associated protein 7 (PAP7) was identified and shown to be involved in hormone-induced steroid biosynthesis in testicular Leydig cells. In the present study, mouse PAP7 cDNA was extended by 5′-rapid amplification of cDNA ends; and a 3432 bp sequence, encoding a 525-amino-acid protein with a calculated molecular weight of 60 kDa, was re-assembled. Mouse and human PAP7 share an 85% amino acid identity and contain a conserved acyl-CoA-binding protein/diazepam binding inhibitor (ACBP/DBI) motif. ACBP/DBI has been identified as the endogenous PBR ligand able to stimulate mitochondrial steroid formation in all steroidogenic cells. The full-length mouse PAP7 gene was cloned and assembled by screening a BAC clone, polymerase chain reaction and searching the mouse genome database. The gene is approximately 29 kb in length and includes eight exons and seven introns. Although it is shorter than the human PAP7 gene, all exons are conserved between the mouse and human. The mouse PAP7 gene was mapped to chromosome 1H3-5 by fluorescence in situ hybridization in agreement with in silico search of the mouse genome database that mapped the PAP7 cDNA sequence to the 1H4 area. Immunofluorescence confocal microscopy demonstrated that PAP7 is mainly localized in the trans-Golgi apparatus and mitochondria in mouse tumor Leydig cells, in agreement with its proposed function in targeting the PKA isoenzyme to organelles rich in PBR, i.e. mitochondria, where phosphorylation of specific protein substrates mediates the hormone-induced steroid formation.
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DOI：
10.1006/bbrc.2001.4975
发表时间：
2001-06-08
影响因子：
3.1
作者：
Lacapère, JJ;Delavoie, F;Vidic, B
通讯作者：
Vidic, B
DOI：
10.1073/pnas.98.3.1267
发表时间：
2001-01
影响因子：
11.1
作者：
Hua Li;Z. Yao;Babett Degenhardt;G. Teper;V. Papadopoulos
通讯作者：
Hua Li;Z. Yao;Babett Degenhardt;G. Teper;V. Papadopoulos
外周苯二氮卓受体结合特性的激素调节是由亚基相互作用介导的。
影响因子：
2.9
作者：
I. Golani;Abraham Weizman;S. Leschiner;I. Spanier;N. Eckstein;R. Limor;J. Yanai;K. Maaser;H. Scherübl;G. Weisinger;M. Gavish
通讯作者：
M. Gavish
Nakamura M.：“平滑肌细胞中血清反应因子和新型 Barx 同源物 Barx1b 介导的 β-原肌球蛋白转录激活”J. Biol。
DOI：
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发表时间：
--
期刊：
影响因子：
--
作者：
通讯作者：
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影响因子：
4
作者：
D. Stocco
通讯作者：
D. Stocco