Global analysis of sumoylation function reveals novel insights into development and appressorium-mediated infection of the rice blast fungus
Global analysis of sumoylation function reveals novel insights into development and appressorium-mediated infection of the rice blast fungus
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苏酰化功能的整体分析揭示了对稻瘟菌发育和附着胞介导的感染的新见解
DOI：
10.1111/nph.15141
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发表时间：
2018-08-01
期刊：
影响因子：
9.4
通讯作者：
Chen, Xiao-Lin
中科院分区：
文献类型：
--
作者：
Liu, Caiyun;Li, Zhigang;Chen, Xiao-Lin
蛋白质翻译后修饰在细胞过程、发育和应激反应中起着关键作用。SUMO修饰是真核生物蛋白质的重要修饰之一，但在植物病原真菌中的功能尚不清楚。本研究结合蛋白质组学、分子生物学和细胞生物学等方法，对SUMO修饰在模式植物病原真菌Magnaporthe中的作用进行了研究，发现SUMO修饰途径在菌落生长中起着重要作用，分生孢子的形成和对宿主的毒力，以及细胞周期相关的表型。类小泛素化也参与对不同胁迫的应答。亲和纯化鉴定了940种推定的SUMO底物，据报道其中许多与发育、应激反应和感染有关。有趣的是，四个septins也被证明是sumoylated。在每个隔蛋白的共识sumoylation位点的突变都导致降低对宿主的毒力和在附着胞内的隔蛋白的错位。此外，SUMO化还参与了不同效应蛋白的胞外分泌，对SUMO化功能的研究为稻瘟病菌的发育和侵染提供了新的视角。
Protein post-translational modifications play critical roles in cellular processes, development and stress response. The small ubiquitin-like modifier (SUMO) to proteins is one of the essential modifications in eukaryotes, but its function remains largely unknown in plant pathogenic fungi.We present a comprehensive analysis combined with proteomic, molecular and cellular approaches to explore the roles of sumoylation in the model plant fungal pathogen, Magnaporthe oryzae.We found the SUMO pathway plays key roles in colony growth, conidia formation and virulence to the host, as well as cell-cycle-related phenotypes. Sumoylation is also involved in responding to different stresses. Affinity purification identified 940 putative SUMO substrates, many of which were reported to be involved in development, stress response and infection. Interestingly, four septins were also shown to be sumoylated. Mutation of consensus sumoylation sites in each septin all resulted in reduced virulence to the host and dislocation of septins in appressoria. Moreover, sumoylation is also involved in extracellular secretion of different effector proteins.Our study on the functions of sumoylation provides novel insight into development and infection of the rice blast fungus.