Method of Noninvasive and Continuous Hemolysis/Thrombogenesis Measurement by Laser Photometry During Artificial Heart Development
Method of Noninvasive and Continuous Hemolysis/Thrombogenesis Measurement by Laser Photometry During Artificial Heart Development
复制标题
人工心脏研制过程中激光光度法无创连续溶血/血栓形成测量方法
DOI：
10.1097/00002480-199709000-00071
复制
发表时间：
1997
期刊：
影响因子：
4.2
通讯作者：
T. Mitsui
中科院分区：
文献类型：
--
作者：
Y. Sankai;T. Tsutsui;T. Jikuya;O. Shigeta;M. Ohta;T. Mitsui
本研究的目的是提出和开发一种方法来测量溶血和血栓形成的非侵入性和连续的，以帮助开发的人工心脏。通常，血红蛋白的光吸收率受氧饱和度的影响，除了在等吸光点处，其不受氧饱和度的影响。因此，作者使用805 nm激光二极管，光谱分析仪，以获得更高的精度。使用Bio-Pump、Tygon管、软壳储液器和光学测量系统构建实验血液回路系统。监测溶血的实验设置如下：血容量200 ml，血流量6 L/min，后负荷200 mmHg。取6次血样（0、30、60、120、180和240 min），并使用比色法测定每次血样中的溶血。将连续激光测量数据与样品数据进行比较，得到了足够的相关性，证明可以连续测量溶血的动态趋势。此外，为了分析血栓形成的过程，使用鱼精蛋白中和的血液进行简单的实验。因此，作者可以看到血栓形成的过程，因为它发生，并可以证实，这种方法能够动态检测溶血和血栓形成。
The purpose of this research is to propose and develop a method to measure hemolysis and thrombogenesis non invasively and continuously to aid in development of an artificial heart. Generally, the optical absorption rate of hemoglobin is influenced by oxygen saturation except at the isosbestic point, which is not influenced by oxygen saturation. The authors, therefore, used an 805 nm laser diode, an optical spectrum analyzer to obtain greater accuracy. An experimental blood circuit system was constructed using a Bio-Pump, Tygon tubing, a soft shell reservoir, and an optical measurement system. Experimental settings for monitoring hemolysis were as follows; blood volume 200 ml, blood flow 6 L/min, and afterload 200 mmHg. Blood was sampled six times (0, 30, 60, 120, 180, and 240 min), and hemolysis in each sampled was measured using a colorimetric method. Comparing continuous laser measurement data with the sample data, an adequate correlation is obtained, proving that the dynamic trend of hemolysis could be continuously measured. Furthermore, to analyze the process of thrombogenesis, simple experiments were performed using blood neutralized by protamine. As a result, the authors could see the process of thrombogenesis as it occurred and could confirm that this method is able to dynamically detect hemolysis and thrombogenesis.