Transformation and recombination in rad mutants of Saccharomyces cerevisiae.
Transformation and recombination in rad mutants of Saccharomyces cerevisiae.
复制标题
酿酒酵母rad突变体的转化和重组。
DOI：
10.1007/bf00265060
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Moore,PD
中科院分区：
文献类型：
--
作者：
Simon,JR;Moore,PD
在转化试验中，检测了酵母RAD 52上位组基因中的破坏/缺失突变对单链和双链环状DNA底物与染色体基因之间重组的影响。在50个突变体中，在leu 2 -3，112等位基因处与单链DNA的重组略有减少;此外，对于单链和双链DNA，几乎完全消除了在trpl-1处的重组。在携带CEN 4的复制质粒上重新引入野生型RAD 50基因，恢复了trpl-1的重组能力，表明RAD 50在该等位基因的基因置换中存在缺陷。与野生型相比，在每个实验中观察到Inrad 52突变体中涉及单链或双链环状DNA的重组减少30%-50%。rad 52突变体中重组的减少与ura 352突变位点的重组相似，ura 352突变位点仅观察到整合事件，而trpl-1突变位点的重组主要通过基因置换发生。无论是therad 54还是therad 57突变对与单链或双链DNA底物的重组都没有显著影响。
Disruption/deletion mutations in genes of theRAD52epistasis group ofSaccharomyces cerevisiaewere examined for their effects on recombination between single-and double-stranded circular DNA substrates and chromosomal genes in a transformation assay. Inrad50mutants there was a small reduction in recombination with single-stranded DNA at theleu2-3, 112allele; in addition there was an almost complete elimination of recombination attrpl-1for both single- and double-stranded DNA. Reintroduction of a wild-typeRAD50gene on a replicating plasmid carryingCEN4restored recombinational competence attrpl-1, indicating thatrad50is defective in gene replacement of this allele. Inrad52mutants a reduction of 30%-50% in recombination involving either single- or double-stranded circular DNA was observed in each experiment when compared to the wild type. This reduction of recombination inrad52mutants was similar for recombination at theura352mutant locus where only integration events have been observed, and at thetrpl-1mutant locus, where recombination occurs predominantly by gene replacement. Neither therad54nor therad57mutations had a significant effect on recombination with single- or double-stranded DNA substrates.