In situ characterization of phytoplankton from vertical profiles of fluorescence emission spectra
In situ characterization of phytoplankton from vertical profiles of fluorescence emission spectra
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根据荧光发射光谱的垂直剖面对浮游植物进行原位表征
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
S. Neuer
中科院分区：
文献类型：
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作者：
T. Cowles;R. Desiderio;S. Neuer
利用激光/光纤荧光计对上层海洋进行垂直剖面图，能够在小的垂直尺度上确定光合色素的荧光发射光谱。同时获取藻红蛋白(PE)和叶绿素(Chl)发射光谱，可以在含有PE的细胞(隐单胞菌和蓝藻)和其他含有Chl的自养生物之间进行原位分化。此外，与不同种类的PE细胞相关的荧光光谱峰移导致了更精细的原位分类分化。我们发现，藻红蛋白的荧光发射最大值从表面附近的578 nm移动到浅温跃层底部的585μm(30%光照水平)，以及真光区底部的590 nm(1%光照水平)。峰值排放的这些变化与聚球藻属更大比例的含有PE的细胞(通过分析离散的瓶子样本确定)的分类变化相吻合。在上层水柱中，真光层底部的隐藻所占比例较大。这些结果表明，浮游植物组合的组成可以在不采集样本的情况下进行现场评估。
Vertical profiling of the upper ocean with a laser/fiber optic fluorometer enabled the determination of fluorescence emission spectra of photosynthetic pigments over small vertical scales. Simultaneous acquisition of phycoerythrin (PE) and chlorophyll (chl) emission spectra allowed in situ differentiation between PE-containing cells (cryptomonads and cyanobacteria) and other chl-containing autotrophs. Further, fluorescence spectral peak shifts associated with different species of PE-containing cells resulted in even finer scale in situ taxonomic differentiation. We found that the phycoerythrin fluorescence emission maxima shifted from 578 nm near the surface, to 585 μm at the base of the shallow thermocline (30% light level), and to 590 nm below the thermocline at the base of the euphotic zone (1% light level). These shifts in peak emission coincided with a taxonomic change in the PE-containing cells (as determined from analysis of discrete bottle samples) from a greater proportion of Synechococcus spp. in the upper water column to a greater proportion of cryptomonads at the base of the euphotic zone. These results indicate that the composition of the phytoplankton assemblage may be assessed in situ without sample collection.