Solubility and Bioavailability of Stabilized Amorphous Calcium Carbonate
Solubility and Bioavailability of Stabilized Amorphous Calcium Carbonate
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DOI：
10.1002/jbmr.196
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发表时间：
2011-02-01
影响因子：
6.2
通讯作者：
Sagi, Amir
中科院分区：
文献类型：
--
作者：
Meiron, Oren E.;Bar-David, Elad;Sagi, Amir
自从大约30年前证实钙在预防人类骨质疏松症中的作用以来，钙的生物利用度一直是许多科学研究的主题。最近的技术允许生产稳定的无定形碳酸钙（ACC），现在可以对这种独特形式的钙进行生物利用度分析。因此，本研究比较了ACC、ACC与壳聚糖（ACC-C）和结晶碳酸钙（CCC）的溶解度和吸收分数。通过将这些制剂溶解在稀磷酸中来评价溶解度。结果表明，ACC和ACC-C都比CCC更易溶解。通过用Ca-45内在标记碳酸钙制剂，使用明胶胶囊经口给予大鼠，评价吸收分数。通过评价血清中测定的每毫升给药放射性剂量的百分比、股骨中的钙吸收和34小时内的全身保留来确定吸收分数。钙血清分析显示，ACC和ACC-C制剂的钙吸收比CCC高40%，而ACC和ACC-C的保留比CCC高26.5%。ACC给药大鼠股骨中吸收的钙比CCC给药动物高30%，而ACC-C给药后吸收的钙比CCC给药后多15%。该研究表明ACC的溶解度和生物利用度优于CCC。根据本研究的结果，建议使用稳定的ACC作为钙的高生物利用度膳食来源。(C)2011年美国骨与矿物质研究学会。
Since its role in the prevention of osteoporosis in humans was proven some 30 years ago, calcium bioavailability has been the subject of numerous scientific studies. Recent technology allowing the production of a stable amorphous calcium carbonate (ACC) now enables a bioavailability analysis of this unique form of calcium. This study thus compares the solubility and fractional absorption of ACC, ACC with chitosan (ACC-C), and crystalline calcium carbonate (CCC). Solubility was evaluated by dissolving these preparations in dilute phosphoric acid. The results demonstrated that both ACC and ACC-C are more soluble than CCC. Fractional absorption was evaluated by intrinsically labeling calcium carbonate preparations with Ca-45, orally administrated to rats using gelatin capsules. Fractional absorption was determined by evaluating the percentage of the administrated radioactive dose per milliliter that was measured in the serum, calcium absorption in the femur, and whole-body retention over a 34-hour period. Calcium serum analysis revealed that calcium absorption from ACC and ACC-C preparations was up to 40% higher than from CCC, whereas retention of ACC and ACC-C was up to 26.5% higher than CCC. Absorbed calcium in the femurs of ACC-administrated rats was 30% higher than in CCC-treated animals, whereas 15% more calcium was absorbed following ACC-C treatment than following CCC treatment. This study demonstrates the enhanced solubility and bioavailability of ACC over CCC. The use of stable ACC as a highly bioavailable dietary source for calcium is proposed based on the findings of this study. (C) 2011 American Society for Bone and Mineral Research.