Sweet taste responses of mouse chorda tympani neurons: existence of gurmarin-sensitive and -insensitive receptor components.
Sweet taste responses of mouse chorda tympani neurons: existence of gurmarin-sensitive and -insensitive receptor components.
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小鼠鼓索神经元的甜味反应：古尔马林敏感和不敏感受体成分的存在。
DOI：
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发表时间：
1999
影响因子：
2.5
通讯作者：
T. Sugimura
中科院分区：
文献类型：
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作者：
Y. Ninomiya;T. Imoto;T. Sugimura
本文观察了古尔马林（gurmarin，gur）对蔗糖等甜味剂诱发的C57 BL小鼠鼓索神经单纤维兴奋性反应的抑制作用。的30个单一的纤维，强烈响应0。5 M蔗糖，但对0.1 M氯化钠、0.01 M盐酸和0.02 M盐酸奎宁没有反应或反应程度较小（蔗糖最好的纤维），16纤维显示出大的抑制反应蔗糖和其他甜味剂舌处理4.8 μ M（约20微克/毫升）gur（抑制至对照的4-52%：gur敏感纤维），而剩余的14根纤维没有显示出这种gur抑制（对照的77-106%：gur不敏感纤维）。在古尔敏感的纤维，蔗糖抑制古尔恢复到约70%的控制响应后，用15 mM β-环糊精冲洗舌头，几乎被废除进一步治疗与2%链霉蛋白酶。在谷氨酰胺不敏感纤维中，蔗糖反应不受谷氨酰胺抑制，但在很大程度上受到链霉蛋白酶抑制。这些结果表明，小鼠味觉细胞中存在两种不同的甜味剂受体成分，一种对gur敏感，另一种对gur不敏感。具有每种成分的味细胞可以由特定类型的鼓索神经元特异性地支配。
Inhibitory effects of gurmarin (gur) on responses to sucrose and other sweeteners of single fibers of the chorda tympani nerve in C57BL mice were examined. Of 30 single fibers that strongly responded to 0. 5 M sucrose but were not or to lesser extent responsive to 0.1 M NaCl, 0.01 M HCl, and 0.02 M quinine HCl (sucrose-best fibers), 16 fibers showed large suppression of responses to sucrose and other sweeteners by lingual treatment with 4.8 microM (approximately 20 microg/ml) gur (suppressed to 4-52% of control: gur-sensitive fibers), whereas the remaining 14 fibers showed no such gur inhibition (77-106% of control: gur-insensitive fibers). In gur-sensitive fibers, responses to sucrose inhibited by gur recovered to approximately 70% of control responses after rinsing the tongue with 15 mM beta-cyclodextrin and were almost abolished by further treatment with 2% pronase. In gur-insensitive fibers, sucrose responses were not inhibited by gur, but were largely suppressed by pronase. These results suggest existence of two different receptor components for sweeteners with different susceptibilities to gur in mouse taste cells, one gur sensitive and the other gur insensitive. Taste cells possessing each component may be specifically innervated by a particular type of chorda tympani neurons.