Evaluation of Antioxidant and Inhibitory Activities for Different Subclasses Flavonoids on Enzymes for Rheumatoid Arthritis
Evaluation of Antioxidant and Inhibitory Activities for Different Subclasses Flavonoids on Enzymes for Rheumatoid Arthritis
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DOI：
10.1111/j.1750-3841.2010.01755.x
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发表时间：
2010-09-10
影响因子：
3.9
通讯作者：
Kim, Gun-Hee
中科院分区：
文献类型：
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作者：
Lee, Je-Hyuk;Kim, Gun-Hee
黄酮类化合物的抗氧化活性依次为黄酮醇类>黄烷酮类>黄酮类。透明质酸酶和胶原酶反应的抑制强度明显不同，根据类黄酮亚类。黄酮醇类化合物中的山奈酚、槲皮素、杨梅苷和芦丁特异性抑制透明质酸酶反应，而黄酮类化合物中的芹菜素、木犀草素、黄芩苷和黄芩素特异性抑制胶原酶反应。除黄芩苷和儿茶素外，黄酮类化合物均能抑制LPS诱导的亚硝酸盐生成，且呈剂量依赖性，这可能主要是通过抑制诱导型一氧化氮合酶（inducible nitric oxide synthase，NO）而实现的。槲皮素和木犀草素对15-脂氧合酶（LOX）的抑制作用最强，槲皮素对环氧合酶-1（考克斯-1）反应的抑制作用相对较强。另外，所有黄酮类化合物对考克斯-2反应均有抑制作用，其中木犀草素、山柰酚、橙皮素和柚皮苷对考克斯-2反应的抑制作用较强。本文阐述了几种黄酮类化合物的抗炎活性，如抗氧化性、抑制NO生成和抑制炎性酶（透明质酸酶、胶原酶、LOX和COX）。
Antioxidant activities of flavonoids were decreased in the order of flavonols > flavanones > flavones. Inhibitory intensities for hyaluronidase and collagenase reaction differed clearly according to flavonoid subclasses. Kaempferol, quercetin, myricetin, and rutin in flavonols inhibited hyaluronidase reaction specifically, while apigenin, luteolin, baicalin, and baicalein in flavones showed specific inhibition to collagenase reaction. In addition, the flavonoids, except baicalin and catechin, inhibited potently LPS-induced nitrite production in a dose-dependent manner, which might be mainly due to the suppression of inducible nitric oxide (NO) synthase. Quercetin and luteolin showed the strongest inhibitory activities on 15-lipoxygenase (LOX), and quercetin showed relatively potent inhibition on cyclooxygenase-1 (COX-1) reaction. Otherwise, all tested flavonoids possessed the inhibitory activity to COX-2 reaction, and especially luteolin, kaempferol, hesperetin, and naringin showed relatively the potent inhibition on COX-2 reaction. This report elucidated the anti-inflammatory activities, such as the antioxidant property, inhibition of NO production, and inhibition of inflammatory enzymes (hyaluronidase, collagenase, LOX, and COXs) of several subclass flavonoids.