H2O2 treatment induces glutathione accumulation and chilling tolerance in mung bean.
H2O2 treatment induces glutathione accumulation and chilling tolerance in mung bean.
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DOI：
10.1071/pp01264
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发表时间：
2002-08
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影响因子：
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通讯作者：
Chih-Wen Yu;T. Murphy;Wei-Wen Sung;Chin-Ho Lin
中科院分区：
文献类型：
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作者：
Chih-Wen Yu;T. Murphy;Wei-Wen Sung;Chin-Ho Lin
绿豆（Vigna radiata L.）CV.以低温敏感品种TN 5为材料，研究谷胱甘肽在过氧化氢（H_2O_2）诱导耐冷性中的作用。在25°C下萌发7 d的种子经受不同时间的低温处理，然后分析其叶片的谷胱甘肽含量。在2-12°C的驯化温度的比较中，发现8°C驯化36小时诱导了5.7倍的增加，这是测试温度中最高的谷胱甘肽水平。在8°C下适应36小时的幼苗在36小时、4°C的低温胁迫后显示出97%的存活率，而非适应植物的存活率为33%。与未处理的对照相比，在36小时、4°C冷却处理之前用200 mM H2 O2预处理12小时，使谷胱甘肽水平增加30%，并使电解质渗漏减少至43%。处理过的幼苗在相同的低温处理后也显示出71%的存活率。应用1 mM的谷胱甘肽合成的特异性抑制剂丁硫氨酸亚砜亚胺，逆转了8°C下驯化36 h或H2 O2预处理对幼苗提供的抗冷胁迫的保护。谷胱甘肽在低温驯化或H2 O2预处理诱导的耐冷性中的作用得到证实。
Mung bean (Vigna radiata L. cv. TN5, a chilling-sensitive cultivar) was employed to evaluate the importance of glutathione in hydrogen peroxide (H2O2)-induced chilling tolerance. Seeds germinated at 25°C for 7d were subjected to different periods of chilling treatment, prior to analysis of the glutathione contents of their leaves. In a comparison of acclimation temperatures from 2-12°C, it was found that an 8°C acclimation for 36 h induced a 5.7-fold increase, the highest glutathione level among the temperatures tested. Seedlings acclimated at 8°C for 36 h showed 97% survival after a 36-h, 4°C chilling stress, compared with 33% survival of non-acclimated plants. Pretreatment with 200 mM H2O2 for 12 h before a 36-h, 4°C chilling treatment increased glutathione levels by 30% and reduced electrolyte leakage to 43%, relative to the untreated control. Treated seedlings also showed a survival rate of 71% after the same chilling treatment. Application of 1 mM buthionine sulfoximine, a specific inhibitor of glutathione synthesis, reversed the protection against chilling stress provided to seedlings either by acclimation at 8°C for 36 h or H2O2 pretreatment. The role of glutathione in chilling acclimation or H2O2-pretreatment-induced chilling tolerance is thus confirmed.