Azoarcus pumilus sp. nov., isolated from seawater in Sanya, China
Azoarcus pumilus sp. nov., isolated from seawater in Sanya, China
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短小固氮弧菌 sp.
DOI：
10.1099/ijsem.0.003341
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发表时间：
2019-05-01
影响因子：
2.8
通讯作者：
Zhang, Xin-qi
中科院分区：
文献类型：
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作者：
Fu, Ge-yi;Yu, Xiao-yun;Zhang, Xin-qi
从三亚海水中分离到一株革兰氏染色阴性、能运动、杆状（0.4-0.5 × 1.0-2.0 μ m）、具有一根极鞭毛的新菌株，命名为SY 39（T）。菌株SY 39（T）能够在15-40 ° C（最适，35-37 ° C）、pH 6.5-8.5（pH 8.0）和0.5- 6.0%（w/v）NaCl（3.5%）下生长。化学分类学分析表明，类异戊二烯醌类化合物为Q-8（88.6%）和Q-7（11.4%）.主要脂肪酸为C-16（：0）和总特征3（C-16（：1）omega 7 c/C-16（：1）omega 6c）。SY 39（T）的极性脂由二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、一种未知的磷酸糖脂、一种未知的糖脂和两种未知的氨基磷酸糖脂组成。基因组DNA的DNA G+C含量为66.5mol%。16 S rRNA基因序列分析表明，菌株SY 39（T）与固氮菌属（Azoarcus）的同源性为92.3%~ 95.2%。根据表型特征、化学分类学特征和系统发育学特征，将菌株SY 39（T）命名为Azoarcuspumilussp. nov.。模式菌株为SY 39（T）（=KCTC 62157（T）= MCCC 1 K 03430（T））。
A novel Gram-stain-negative, motile, rod-shaped (0.4-0.5 x 1.0-2.0 mu m) strain with one polar flagellum, designated SY39(T), was isolated from seawater in Sanya, China. Strain SY39(T) was able to grow at 15-40 degrees C (optimum, 35-37 degrees C), pH 6.5-8.5 (pH 8.0) and 0.5-6.0 % (w/v) NaCl (3.5 %). Chemotaxonomic analysis showed that the isoprenoid quinones were Q-8 (88.6 %) and Q-7 (11.4 %). The dominant fatty acids were C-16(:0) and summed feature 3 (C-16(:1)omega 7c/C-16(:1)omega 6c). The polar lipids of strain SY39(T) consisted of diphosphatidyglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unknown phosphoglycolipid, one unknown glycolipid and two unknown aminophosphoglycolipids. The DNA G+C content of the genomic DNA was 66.5 mol%. The phylogenetic analysis of 16S rRNA gene sequences showed that strain SY39(T) belongs to the genus Azoarcus with similarity ranging from 92.3 to 95.2 %. Based on the phenotypic, chemotaxonomic and phylogenetic features, strain SY39(T) is concluded to represent a novel species of the genus Azoarcus, for which the name Azoarcus pumilus sp. nov. is proposed. The type strain is SY39(T) (=KCTC 62157(T) = MCCC 1K03430(T)).