MYELOID-LEUKEMIA DIFFERENTIATION BY PHORBOL ESTER AND RETINOIC ACID - A PRACTICAL APPROACH
MYELOID-LEUKEMIA DIFFERENTIATION BY PHORBOL ESTER AND RETINOIC ACID - A PRACTICAL APPROACH
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DOI：
10.1002/jcla.1860040506
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发表时间：
1990-01-01
影响因子：
2.7
通讯作者：
RUMJANEK, VM
中科院分区：
文献类型：
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作者：
MARQUESSILVA, VM;DESOUZA, MHO;RUMJANEK, VM
本文观察了TPA（12-O-十四烷酰基-佛波醇-13-乙酸酯）和RA（维甲酸）对HL 60（急性早幼粒细胞白血病）和K 562（红白血病）细胞系及几种白血病患者细胞的作用。急性淋巴细胞白血病（ALL）14例，慢性淋巴细胞白血病（CLL）2例，急性髓细胞白血病（CLL）23例，急性髓细胞白血病（MI-M7）23例，慢性粒细胞白血病急变（CML-BC）5例，混合性白血病2例。在几乎所有检查的病例中，在TPA暴露后，来自被证实患有髓性白血病的患者的细胞粘附于基质，而淋巴样白血病细胞保持在悬浮液中，从而允许淋巴样细胞从髓性母细胞分化。唯一的例外是在一个CLL的情况下，它的细胞变得粘附与长的视网膜突出。此外，TPA暴露后吞噬作用增加允许表征M7，因为这仅是吞噬作用阴性的髓性白血病。此外，髓性白血病的亚型之间的区分可以是髓性白血病的亚型可以基于在M4和M5中TPA后观察到的增加的溶菌酶产生。酯酶阳性可以区分M1细胞，TPA处理前后均为阴性。与其他作者的结果一致，TPA和RA导致了独立的分化方式，RA和TPA分别有利于粒细胞样谱系和单核细胞样细胞。同一细胞分化成一种以上谱系的能力，取决于是否使用RA或TPA，仅在本研究中用M3细胞观察到。我们的研究结果表明，使用分化诱导剂和功能测试与形态学一起阐明白血病细胞的性质是有用的。
The effects of TPA (12-O-tetradecanoyl-phorbol-13-acetate) and RA (retinoic acid) were investigated on the cell lines HL60 (acute promyelocytic leukemia) and K562 (erythroleukemia) and on cells from patients with several kinds of leukemia. There were 14 cases of acute lymphocytic leukemia (ALL), 2 cases of chronic lymphocytic leukemia (CLL), 23 cases of acute myeloid leukemia (CLL), 23 cases of acute myeloid leukemia (MI-M7), 5 cases of chronic myelocytic leukemia in blast crisis (CML-BC) and 2 mixed leukemias. In almost all of the cases examined, after TPA exposure cells from patients with proven myeloid leukemia became adherent to the substrate, while lymphoid leukemia cells remained in suspension, allowing the differentiation of lymphoid from myeloid blasts. The only exception was in one case of CLL, which had cells that became adherent with long filamental projections. In addition, increased phagocytosis following TPA exposure permitted characterization of M7 as this was only myeloid leukemia negative for phagocytosis. Furthermore discrimination between the subtypes of myeloid leukemia could be subtypes of myeloid leukemia could be based on the increased lysozyme production seen after TPA in M4 and M5. Esterase positivity allowed the discrimination of M1 cells, which were negative before and after TPA treatment. In agreement with the results of other authors, TPA and RA led to independent ways of differentiation, granulocytic-like lineage and monocytic-like cells being favored by RA and TPA, respectively. The capacity of the same cell to differentiate into more than one lineage, depending on whether RA or TPA was used, was only seen in the present study with M3 cells. Our results show the usefulness of using differentiation inducers and functional tests together with morphology to elucidate the nature of leukemic cells.