MALDI-based imaging mass spectrometry revealed abnormal distribution of phospholipids in colon cancer liver metastasis
MALDI-based imaging mass spectrometry revealed abnormal distribution of phospholipids in colon cancer liver metastasis
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DOI：
10.1016/j.jchromb.2007.02.037
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发表时间：
2007-08-01
影响因子：
3
通讯作者：
Setou, Mitsutoshi
中科院分区：
文献类型：
--
作者：
Shimma, Shuichi;Sugiura, Yuki;Setou, Mitsutoshi
我们介绍了基质辅助激光解吸/电离(MALDI)成像和串联质谱仪(MS/MS)在结肠癌肝转移中的直接分子鉴定结果。从一名日本患者身上取出了癌症组织，并立即冷冻，没有任何固定。切片厚度为3微米，脂质电离基质为2，6-二羟基苯乙酮。用喷枪将基质溶液涂抹到组织表面的薄而均匀的基质层中。在二维激光扫描之后，从数百个获得的光谱中重建作为m/z的函数的图像。在获得的图像中，分子的存在由与信号强度相对应的伪彩色来表示。在一项可行性研究中，我们在一个癌变区域接收到了一个局部信号，m/z 725。MS/MS结果表明m/z 725为鞘磷脂(16：0)+Na。因此，我们成功地证明了MALDI成像作为一种分析病理标本的工具的可行性。(C)2007 Elsevier B.V.保留所有权利。
We present the results of matrix-assisted laser desorption/ionization (MALDI) imaging and direct molecular identification using tandem mass spectrometry (MS/MS) in colon cancer liver metastasis. Cancer tissue was removed from a Japanese patient and frozen immediately without any fixations. The sections were sliced to a thickness of 3 mu m. The matrix for lipid ionization was 2,6-dihydroxy acetophenone. The matrix solution was applied with an airbrush into a thin uniform matrix layer on the tissue surface. After two-dimensional laser scanning, the images were reconstructed as a function of m/z from a few hundred obtained spectra. In the obtained images, the existence of molecules was represented by a pseudo-color corresponding to the signal intensity. In a feasibility study, we picked up a localized signal, m/z 725 in a cancerous area. The MS/MS result suggested that m/z 725 was sphingomyelin(16:0)+Na. Thus, we successfully show the feasibility of MALDI imaging as a tool for the analysis of pathological specimens. (C) 2007 Elsevier B.V. All rights reserved.