A molecular approach to the concerted action of kinases involved in energy homoeostasis.
A molecular approach to the concerted action of kinases involved in energy homoeostasis.
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参与能量稳态的激酶协同作用的分子方法。
DOI：
10.1042/bst0310169
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发表时间：
2001
影响因子：
3.9
通讯作者：
T. Wallimann
中科院分区：
文献类型：
--
作者：
D. Neumann;U. Schlattner;T. Wallimann
细胞最重要的职责之一是能量稳态。几种激酶，包括AMP活化蛋白激酶（AMPK），肌酸激酶和腺苷酸激酶，参与对应激的即时反应，导致能量消耗。在这里，我们提出了我们的看法之前的下游过程介导的AMPK，并导致减少能量消耗和增加能量生产的事件。不幸的是，AMPK在分子水平上的定义非常差。因此，提出了一种以毫克量生产AMPK的方法，这将极大地促进这种蛋白激酶的功能和结构表征。
One of the most important duties of a cell is energy homoeostasis. Several kinases, including AMP-activated protein kinase (AMPK), creatine kinase and adenylate kinase, are involved in the immediate response to stress, resulting in energy depletion. Here, we present our view of events preceding the downstream processes mediated by AMPK and leading to reduced energy expenditure and increased energy production. Unfortunately, AMPK is very poorly defined at the molecular level. Thus a procedure for production of AMPK in milligram amounts is presented which will greatly facilitate the functional and structural characterization of this protein kinase.