Transforming growth factors produced by retrovirus-transformed rodent fibroblasts and human melanoma cells: amino acid sequence homology with epidermal growth factor.
Transforming growth factors produced by retrovirus-transformed rodent fibroblasts and human melanoma cells: amino acid sequence homology with epidermal growth factor.
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DOI：
10.1073/pnas.80.15.4684
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发表时间：
1983-08
影响因子：
11.1
通讯作者：
H. Marquardt;M. Hunkapiller;Leroy Hood;D. Twardzik;J. D. Larco;J. Stephenson;G. Todaro
中科院分区：
文献类型：
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作者：
H. Marquardt;M. Hunkapiller;Leroy Hood;D. Twardzik;J. D. Larco;J. Stephenson;G. Todaro
转化生长因子（TGF）纯化无血清培养基条件逆转录病毒转化的费舍尔大鼠胚胎成纤维细胞，小鼠3 T3细胞，和两个人黑色素瘤细胞系。每个TGF的纯化进行监测，在放射受体测定的基础上与小鼠颌下腺表皮生长因子（mEGF）的受体交叉反应性，并实现了通过凝胶渗透色谱法的酸溶性TGF-含活性，然后通过反相高压液相色谱法，依次使用乙腈和1-丙醇在三氟乙酸水溶液的存在下。测定大鼠、小鼠和人TGF的氨基末端序列。在来自不同物种和细胞类型的TGF多肽之间发现了广泛的序列同源性。大鼠TGF、mEGF和人尿抑胃素（hEGF）的氨基酸序列的比对揭示了统计学上显著的序列同源性。报道的结果表明，竞争结合细胞EGF受体和EGF的TGF可能是从共同的祖先进化而来的。
Transforming growth factors (TGFs) were purified from serum-free medium conditioned by retrovirus-transformed Fisher rat embryo fibroblasts, mouse 3T3 cells, and two human melanoma cell lines. The purification of each TGF was monitored in a radioreceptor assay based on receptor crossreactivity with mouse submaxillary gland epidermal growth factor (mEGF) and was achieved by gel permeation chromatography of the acid-soluble TGF-containing activity, followed by reverse-phase high-pressure liquid chromatography with sequential use of acetonitrile and 1-propanol in the presence of aqueous trifluoroacetic acid. The amino-terminal sequences of rat, mouse, and human TGFs were determined. Extensive sequence homology was found among TGF polypeptides from different species and cell types. Alignment of the amino acid sequences of rat TGF, mEGF, and human urogastrone (hEGF) reveals statistically significant sequence homology. The reported results suggest that TGFs that compete for binding to the cellular EGF receptor and EGF may have evolved from a common progenitor.