Hydrogen-rich water alleviates aluminum-induced inhibition of root elongation in alfalfa via decreasing nitric oxide production
Hydrogen-rich water alleviates aluminum-induced inhibition of root elongation in alfalfa via decreasing nitric oxide production
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DOI：
10.1016/j.jhazmat.2013.12.029
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发表时间：
2014-02-28
影响因子：
13.6
通讯作者：
Shen, Wenbiao
中科院分区：
文献类型：
--
作者：
Chen, Meng;Cui, Weiti;Shen, Wenbiao
铝（Al）中毒最早和最明显的症状之一是抑制根伸长。虽然氢气（H-2）最近被描述为植物中重要的生物调节剂，但H-2是否以及如何调节铝诱导的根伸长抑制在很大程度上是未知的。为了解决这些空白，利用富氢水（HRW）研究了H2的生理作用及其可能的分子机制。单独或同时（特别是）暴露于Al或新鲜而不陈旧的一氧化氮（NO）释放化合物硝普钠（SNP），不仅增加了NO的产生，而且显著抑制了根系伸长。在HRW饱和度达到50%的情况下，上述反应得到了不同程度的缓解。HRW的加入也缓解了Al毒性症状的出现，包括改善幼苗生长和减少Al的积累。随后的研究结果表明，NO清除剂去除NO，与HRW类似，可以减少NO的产生，减轻Al或snp诱导的根生长抑制。因此，我们认为HRW通过降低NO的产生来缓解al诱导的苜蓿根伸长抑制。这些发现可能适用于提高作物产量和提高抗逆性。(C) 2013 Elsevier B.V.版权所有
One of the earliest and distinct symptoms of aluminum (Al) toxicity is the inhibition of root elongation. Although hydrogen gas (H-2) is recently described as an important bio-regulator in plants, whether and how H-2 regulates Al-induced inhibition of root elongation is largely unknown. To address these gaps, hydrogen-rich water (HRW) was used to investigate a physiological role of H2 and its possible molecular mechanism. Individual or simultaneous (in particular) exposure of alfalfa seedlings to Al, or a fresh but not old nitric oxide (NO)-releasing compound sodium nitroprusside (SNP), not only increased NO production, but also led to a significant inhibition of root elongation. Above responses were differentially alleviated by pretreatment with 50% saturation of HRW. The addition of HRW also alleviated the appearance of Al toxicity symptoms, including the improvement of seedling growth and less accumulation of Al. Subsequent results revealed that the removal of NO by the NO scavenger, similar to HRW, could decrease NO production and alleviate Al- or SNP-induced inhibition of root growth. Thus, we proposed that HRW alleviated Al-induced inhibition of alfalfa root elongation by decreasing NO production. Such findings may be applicable to enhance crop yield and improve stress tolerance. (C) 2013 Elsevier B.V. All rights reserved.