Exiguobacterium profundum sp nov., a moderately thermophilic, lactic acid-producing bacterium isolated from a deep-sea hydrothermal vent
Exiguobacterium profundum sp nov., a moderately thermophilic, lactic acid-producing bacterium isolated from a deep-sea hydrothermal vent
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DOI：
10.1099/ijs.0.64639-0
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发表时间：
2007-02-01
影响因子：
2.8
通讯作者：
Combet-Blanc, Yannick
中科院分区：
文献类型：
--
作者：
Crapart, Sylvaine;Fardeau, Marie-Laure;Combet-Blanc, Yannick
从东太平洋海隆北纬 13 度、深度约 2600 m 处采集的深海热液喷口样本中分离出一种兼性厌氧、耐盐、中度嗜热且不产生孢子的细菌，命名为菌株 10C(T)。菌株10C(T)的细胞是革兰氏阳性、能动的杆状细胞，并且在45℃（范围12-49℃）、pH 7.0（范围pH 5.5-9.5）和0-2% NaCl（范围0-11%）下生长最佳。 (+)-L-乳酸是从碳水化合物发酵中检测到的主要有机酸，并含有痕量的甲酸盐、乙酸盐和乙醇。菌株10C(T)是过氧化氢酶阳性、氧化酶阴性并且在厌氧条件下将硝酸盐还原为亚硝酸盐。 DNA G+C含量为50.4mol%。其最接近的系统发育亲属是河口微小杆菌 TF-16(T) 和海洋微小杆菌 TF-80(T)（16S rRNA 基因序列相似性 > 99%）。然而，如 DNA-DNA 相关性数据所示，菌株 10C(T) 在基因型上与这两个微小杆菌属物种不同。因此，根据表型、基因型和系统发育特征，菌株10C(T)被认为代表了微小杆菌属的一个新种，其名称为Exiguobacter profundum, sp.。十一月被提议。典型菌株为 10C(T) (=CCUG 50949(T) = DSM 172E39(T))。
A facultatively anaerobic, halotolerant, moderately thermophilic and non-sporulating bacterium, designated strain 10C(T), was isolated from deep-sea hydrothermal vent samples collected on the 13 degrees N East Pacific Rise at a depth of approximately 2600 m. Cells of strain 10C(T) were Grampositive, motile rods, and grew optimally at 45 degrees C (range 12-49 degrees C), pH 7.0 (range pH 5.5-9.5) and 0-2 % NaCl (range 0-11 %). (+)-L-Lactate was the main organic acid detected from carbohydrate fermentation with traces of formate, acetate and ethanol. Strain 10C(T) was catalasepositive, oxidase-negative and reduced nitrate to nitrite under anaerobic conditions. The DNA G + C content was 50.4 mol%. Its closest phylogenetic relatives were Exiguobacterium aestuarii TF-16(T) and Exiguobacterium marinum TF-80(T) (16S rRNA gene sequence similarity > 99 %). However, strain 10C(T) differed genotypically from these two Exiguobacterium species as indicated by DNA-DNA relatedness data. Therefore, on the basis of phenotypic, genotypic and phylogenetic characteristics, strain 10C(T) is considered to represent a novel species of the genus Exiguobacterium, for which the name Exiguobacterium profundum, sp. nov. is proposed. The type strain is 10C(T) (=CCUG 50949(T) = DSM 172E39(T)).