The mitogenic activity of insulin: an intrinsic property of the molecule.
The mitogenic activity of insulin: an intrinsic property of the molecule.
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胰岛素的促有丝分裂活性：分子的固有特性。
DOI：
10.1016/0006-291x(80)91591-0
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发表时间：
1980
影响因子：
3.1
通讯作者：
Böhlen,P
中科院分区：
文献类型：
--
作者：
Petrides,PE;Böhlen,P
高于生理浓度的胰岛素可刺激培养的大量细胞中[3H]胸苷掺入DNA。为了排除导致这种促有丝分裂作用的胰岛素制剂污染物，通过反相HPLC纯化猪胰岛素并测定促有丝分裂活性。HPLC纯化的胰岛素具有与不纯胰岛素相似的促有丝分裂活性。此外，半合成和合成胰岛素以及HPLC纯化的胰岛素衍生物，如单脱酰胺和单精氨酸胰岛素也具有生物活性。
Higher than physiological concentrations of insulin stimulate the incorporation of [3 H] thymidine into DNA in a large variety of cells in culture. In order to exclude a contaminant of insulin preparations as responsible for this mitogenic action, porcine insulin was purified by reverse phase HPLC and assayed for mitogenic activity. HPLC-purified insulin had a mitogenic activity similar to that of impure insulin. Moreover, semisynthetic and synthetic insulins as well as HPLC-purified insulin derivatives such as monodesamido-and monoarginine insulin were also biologically active.