Global gene expression changes in human peripheral blood after H7N9 infection
Global gene expression changes in human peripheral blood after H7N9 infection
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DOI：
10.1016/j.gene.2014.08.062
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发表时间：
2014-11-10
期刊：
影响因子：
3.5
通讯作者：
Wang, Mei-tang
中科院分区：
文献类型：
--
作者：
Mei, Bing;Ding, Xian;Wang, Mei-tang
2013年，中国东部出现了一种新型的人感染禽流感病毒（H7N9），导致轻度至致命的人呼吸道感染。然而，其潜在的分子机制在很大程度上仍然未知。在这项工作中，我们试图在转录水平上深入了解这种疾病的潜在遗传基础。我们收集了H7N9感染患者和健康人的外周血样本，然后进行转录组分析，以全面研究其表达特征，这将有助于我们更好地了解病毒感染后病原学的分子基础。通过对H7N9病毒感染和未感染的样本进行高通量RNA-seq分析，我们总共鉴定了1091个显著差异表达的基因。在差异表达基因中发现了与免疫和炎症反应相关的多条生物学通路。对H7N9病毒携带者和健康人之间基因调控的全基因组筛查为理解和应对这种潜在威胁提供了一些见解。(C)2014爱思唯尔有限公司版权所有。
A novel avian influenza A virus (H7N9) of human infection emerged in eastern China in 2013, causing mild to lethal human respiratory infections. However, the underlying molecular mechanism remains largely unknown. In this work, we attempt to gain insights into the underlying genetic basis of this disease at the transcription level. We collected peripheral blood samples from patients with H7N9 infection and healthy people, and then we performed transcriptome profiling to comprehensively investigate their expression signatures, which would help us to better understand the molecular basis of the etiology upon viral infection. By employing the high throughput RNA-seq analysis of samples with and without H7N9 viral infection, we totally identified 1091 significantly differentially expressed genes. We found that several biological pathways related to the immunity and inflammation response in the differentially expressed genes. A genome-wide screening of gene regulation between H7N9 virus carrier and healthy people provided some insights into understanding and responsiveness to this potential threat. (C) 2014 Elsevier B.V. All rights reserved.