The genome of Erwinia tasmaniensis strain Et1/99, a non-pathogenic bacterium in the genus Erwinia
The genome of Erwinia tasmaniensis strain Et1/99, a non-pathogenic bacterium in the genus Erwinia
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DOI：
10.1111/j.1462-2920.2008.01639.x
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发表时间：
2008-09-01
影响因子：
5.1
通讯作者：
Geider, Klaus
中科院分区：
文献类型：
--
作者：
Kube, Michael;Migdoll, Alexander Michael;Geider, Klaus
对塔斯马尼亚Erwinia tasmaniensis菌株Et1/99的全基因组进行了测序，其中包括一条3.9 Mb的环状染色体和5个质粒。菌株Et1/99是一种与淀粉Erwinia amylovora和梨叶Erwinia pyrifoliae有亲缘关系的附生植物细菌，它们分别是造成亚洲梨枝枯病和火枯病的重要植物病害。菌株Et1/99是一种非致病性细菌，被认为在最初定植期间与这些细菌和其他细菌占据相同的栖息地时竞争。基因组分析揭示了定植、细胞通讯和防御调节的工具，以及编码levan合成和未检测到的荚膜外多糖的基因。菌株Et1/99可能分泌吲哚-3-乙酸，以增加植物表面提供的营养物质的有效性。这些营养物质随后被获取和代谢。分泌系统包括许多病原体中存在的超敏反应III型途径。菌株Et1/99与Pectobacterium atrosepticum及其相关的Erwinia spp等病原体之间存在毒力相关因子的差异或缺失部分，菌株Et1/99完全缺乏山梨醇操纵子，这也可能影响其无法入侵火疫病主植物。相比之下，淀粉欧文菌的毒力取决于对山梨糖醇的利用，山梨糖醇是蔷薇科植物中主要的碳水化合物。菌株Et1/99中其他毒力相关因子的存在表明许多植物相关细菌的祖先基因组背景。
The complete genome of the bacterium Erwinia tasmaniensis strain Et1/99 consisting of a 3.9 Mb circular chromosome and five plasmids was sequenced. Strain Et1/99 represents an epiphytic plant bacterium related to Erwinia amylovora and E. pyrifoliae, which are responsible for the important plant diseases fire blight and Asian pear shoot blight, respectively. Strain Et1/99 is a non-pathogenic bacterium and is thought to compete with these and other bacteria when occupying the same habitat during initial colonization. Genome analysis revealed tools for colonization, cellular communication and defence modulation, as well as genes coding for the synthesis of levan and a not detected capsular exopolysaccharide. Strain Et1/99 may secrete indole-3-acetic acid to increase availability of nutrients provided on plant surfaces. These nutrients are subsequently accessed and metabolized. Secretion systems include the hypersensitive response type III pathway present in many pathogens. Differences or missing parts within the virulence-related factors distinguish strain Et1/99 from pathogens such as Pectobacterium atrosepticum and the related Erwinia spp. Strain Et1/99 completely lacks the sorbitol operon, which may also affect its inability to invade fire blight host plants. Erwinia amylovora in contrast depends for virulence on utilization of sorbitol, the dominant carbohydrate in rosaceous plants. The presence of other virulence-associated factors in strain Et1/99 indicates the ancestral genomic background of many plant-associated bacteria.