Increase of insulin secretion by ginsenoside Rh2 to lower plasma glucose in wistar rats
Increase of insulin secretion by ginsenoside Rh2 to lower plasma glucose in wistar rats
复制标题
DOI：
10.1111/j.1440-1681.2006.04319.x
复制
发表时间：
2006-01-01
影响因子：
2.9
通讯作者：
Cheng, JT
中科院分区：
文献类型：
--
作者：
Lee, WK;Kao, ST;Cheng, JT
1.本研究的目的是阐明人参根中降血糖活性成分α Rh 2的作用.使用葡萄糖氧化酶法评估血浆葡萄糖。用ELISA法测定血浆胰岛素和C肽水平的变化.禁食Wistar大鼠静脉注射0.1- 1.0mg/kg的Rh 260 min后，血糖呈剂量依赖性下降。在接受相同给药的大鼠中，观察到与血糖降低平行的血浆胰岛素水平以及血浆C肽升高。Rh 2的这些作用可被阿托品（0.1-1.0 mg/kg）逆转，但不受神经节烟碱拮抗剂喷托溴铵或六甲双铵（均为7.5 mg/kg）的影响.使用胆碱摄取抑制剂（hemicholinium-3; 1-10 mg/kg）或囊泡乙酰胆碱转运抑制剂（vesamicol; 1.5-3.5 mg/kg）破坏突触可用乙酰胆碱（ACh）可消除Rh 2的作用。此外，毒扁豆碱（0.1-0.5毫克/公斤），在足以抑制乙酰胆碱酯酶的浓度，增强的行动，AIR-Rh 2。因此，可以考虑通过从神经末梢释放的ACh来介导Rh 2增强胰岛素分泌的作用. 4-二苯乙酰氧基-N-甲基哌啶碘甲烷（4-DAMP; 5-10 mg/kg）阻断血浆胰岛素的增加和Rh 2的血浆降糖作用表明毒蕈碱M-3受体的参与。Rh 2诱导的血浆C肽水平升高对4-DAMP也敏感。本研究的结果表明，ACh Rh 2具有增加胰岛素分泌的能力，这是由于ACh从神经末梢释放，然后刺激胰腺细胞中的M-3受体。这一发现显示了人参皂苷Rh 2的降血糖作用机制，人参皂苷Rh 2是人参根中所含的主要成分之一。因此，可将CD 3Rh 2用作糖尿病管理的佐剂。
1. The aim of the present study was to clarify the role of ginsenoside Rh2 as the active compound in Panax ginseng root for lowering plasma glucose in animals.2. Plasma glucose was assessed using the glucose oxidase method. Changes in the levels of insulin and C-peptide in plasma were measured by ELISA using commercially available kits.3. After intravenous injection into fasting Wistar rats for 60 min, ginsenoside Rh2 (0.1-1.0 mg/kg) decreased plasma glucose in a dose-dependent manner. In parallel with the decrease in plasma glucose, increases in plasma insulin levels, as well as plasma C-peptide, were observed in rats receiving the same treatment. These effects of Rh2 were reversed by atropine (0.1-1.0 mg/kg), but not affected by the ganglionic nicotinic antagonists pentolinium or hexamethonium (both at 7.5 mg/kg).4. Disruption of synaptically available acetylcholine (ACh) using an inhibitor of choline uptake (hemicholinium-3; 1-10 mg/kg) or an inhibitor of vesicular ACh transport (vesamicol; 1.5-3.5 mg/kg) abolished the actions of Rh2. In addition, physostigmine (0.1-0.5 mg/kg), at a concentration sufficient to inhibit acetylcholinesterase, enhanced the actions of the ginsenoside Rh2. Thus, mediation of the effects of Rh2 to enhance insulin secretion by ACh released from nerve terminals can be considered.5. Blockade of the increase in plasma insulin and the plasma glucose-lowering action of Rh2 by 4-diphenylacetoxy-N-methylpiperdine methiodide (4-DAMP; 5-10 mg/kg) indicates the participation of muscarinic M-3 receptors. Increases in plasma C-peptide level induced by Rh2 were also sensitive to 4-DAMP.6. The results of the present study suggest that ginsenoside Rh2 has the ability to increase insulin secretion as a result of the release of ACh from nerve terminals that then stimulates muscarinic M-3 receptors in pancreatic cells. This finding shows the mechanism for the plasma glucose-lowering action of ginsenoside Rh2, that is one of the major principles contained in P. ginseng root. Thus, ginsenoside Rh2 may be applied as an adjuvant for the management of diabetes.