Molecular characterization of ochratoxin A producing strains of the genus Penicillium
Molecular characterization of ochratoxin A producing strains of the genus Penicillium
复制标题
DOI：
10.1078/0723-2020-00094
复制
发表时间：
2002-04-01
影响因子：
3.4
通讯作者：
Geisen, R
中科院分区：
文献类型：
--
作者：
Castella, G;Larsen, TO;Geisen, R
采用RAPD、AFLP和ITS序列分析等分子生物学方法对66株根据油脂形态学标准划分为疣孢青霉的菌株进行了鉴定。RAPD和AFLP分析可将其分为两个类群。两个RAPD和两个AFLP组完全吻合。这两组菌株的产赭曲霉毒素A能力不同，第一组主要是高产菌株，第二组是中等至不产菌株。来自组I的菌株来源于食物，如奶酪和肉制品，而来自组II的菌株来源于植物。核糖体ITS 1 -5. 8 S-ITS 2序列除两个单核苷酸交换外，其余序列相似，部分菌株的化学分类学分析表明，各组间次级代谢产物的产生存在差异。I组菌株具有P. nordicum的化学型，II组菌株具有P. verrucosum的化学型。RAPD和AFLP水平的差异与化学型差异平行，与最近将产赭曲霉毒素A的青霉重新分类为疣状青霉或诺氏青霉一致。ITS序列的同源性表明了两个种之间的系统发育关系。
Sixty-six strains classified as P. verrucosum based oil morphological criteria were characterized by molecular methods like RAPD, AFLP and ITS sequencing. Two groups could be identified by RAPD and AFLP analyses. The two RAPD as well as the two AFLP groups were completely coincidental. Strains ill the two groups differed in their ability to produce ochratoxin A, with group I containing mainly high producing strains, and group II containing moderate to non-producing strains. The strains from group I originate from foods, such as cheeses and meat products, while the strains from group II originate from plants. The ribosomal ITS1-5.8S-ITS2 sequences were similar, except for two single nucleotide exchanges in several strains of each group.A chemotaxonomical analysis of some of the strains identified differences between the groups in secondary metabolite production. Strains from group I possessed the chemotype of P. nordicum and strains from group II that of P. verrucosum. The differences at the RAPD and AFLP level, which parallel the chemotypic differences, are consistent with the recent reclassification of ochratoxin A producing penicillia to be either P. verrucosum or P. nordicum. The homolgy between the ITS sequences however indicates phylogenetic relationship between the two species.