Molecular cloning, structural characterization and functional expression of the human substance P receptor.
Molecular cloning, structural characterization and functional expression of the human substance P receptor.
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DOI：
10.1016/0006-291x(91)91704-g
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发表时间：
1991-09
影响因子：
3.1
通讯作者：
Yasuo Takeda;K. B. Chou;J. Takeda;B. Sachais;J. E. Krause
中科院分区：
文献类型：
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作者：
Yasuo Takeda;K. B. Chou;J. Takeda;B. Sachais;J. E. Krause
分离到了一段编码人P物质受体（SPR）的cDNA，并通过核苷酸序列分析推断出该蛋白的一级结构。该SPR由407个残基组成，是g蛋白偶联受体超家族的成员。大鼠SPR序列与人SPR序列的一致性为94.5%。该受体在COS-7细胞系中表达，对Tyr−1-SP的结合kd值为0.24 nM。由自然产生的速激肽引起的配体位移是SP > neurokinin A > neurokinin B. SP刺激转染细胞导致快速和短暂的肌醇1,4,5-三磷酸反应。RNA印迹杂交和溶液杂交表明，SPR mRNA的大小约为4.5 Kb，在IM-9淋巴母细胞和U373-MG星形细胞瘤细胞以及脊髓和肺中表达，但在肝脏中不表达。
A cDNA encoding the human substance P receptor (SPR) was isolated and the primary structure of the protein was deduced by nucleotide sequence analysis. This SPR consists of 407 residues and is a member of the G-protein coupled receptor superfamily. Comparison of rat and human SPR sequences demonstrated a 94.5% identity. The receptor was expressed in a COS-7 cell line and displayed a Kdfor Tyr−1-SP binding of 0.24 nM. Ligand displacement by naturally occurring tachykinin peptides was SP ≫ neurokinin A > neurokinin B. SP stimulation of transfected cells resulted in a rapid and transient inositol 1,4,5-trisphosphate response. RNA blot hybridization and solution hybridization demonstrated that SPR mRNA was about 4.5 Kb in size, and was expressed in IM-9 lymphoblast and U373-MG astrocytoma cells, as well as in spinal cord and lung but not in liver.