Green tea diet decreases PCB 126-induced oxidative stress in mice by up-regulating antioxidant enzymes.
Green tea diet decreases PCB 126-induced oxidative stress in mice by up-regulating antioxidant enzymes.
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DOI：
10.1016/j.jnutbio.2013.10.003
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发表时间：
2014-02
期刊：
影响因子：
--
通讯作者：
Hennig B
中科院分区：
文献类型：
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作者：
Newsome BJ;Petriello MC;Han SG;Murphy MO;Eske KE;Sunkara M;Morris AJ;Hennig B
超级基金化学品，如多氯联苯，由于其环境持久性和与多种疾病有关，对人类健康构成严重威胁。有选择性的生物活性食品成分，如类黄酮，已被证明可以减轻多氯联苯的毒性，但主要是在体外环境中。在这里，我们表明，喂食绿茶强化饮食并随后暴露于环境相关剂量的共面多氯联苯的小鼠表现出总体氧化应激降低，这主要是由于一组抗氧化酶的上调。C57BL/6小鼠饲喂含绿茶提取物的低脂饲料12周，分别于第10、11、12周给予5μ摩尔PCB126/kg体重(1.6 3 mg/kg·d)染毒(总体重4.9 mg/kg)。F2-异前列腺素及其代谢物是体内氧化应激的公认标记物，通过高效液相-质谱仪/质谱仪在血浆中测量，结果显示，补充GTE并随后暴露于多氯联苯的小鼠的水平比暴露于多氯联苯的对照饮食的小鼠降低了五倍。收集并收获肝脏进行mRNA和蛋白质分析，结果表明，在喂食绿茶的多氯联苯暴露的小鼠中，许多由AhR和Nrf2蛋白转录控制的基因上调。在这些小鼠(绿茶加多氯联苯)中，SOD1、GSR、NQO1和GST等关键抗氧化酶基因的诱导增加可能解释了观察到的总体氧化应激的下降。饮食中添加绿茶可以在多氯联苯126存在的情况下实现有效的抗氧化反应，这支持了一种新兴的范式，即健康的营养可能能够支持和缓冲生理系统免受环境污染物的毒性。
Superfund chemicals such as polychlorinated biphenyls pose a serious human health risk due to their environmental persistence and link to multiple diseases. Selective bioactive food components such as flavonoids have been shown to ameliorate PCB toxicity, but primarily in an in vitro setting. Here, we show that mice fed a green tea-enriched diet and subsequently exposed to environmentally relevant doses of coplanar PCB exhibit decreased overall oxidative stress primarily due to the upregulation of a battery of antioxidant enzymes. C57BL/6 mice were fed a low fat diet supplemented with green tea extract (GTE) for 12 weeks and exposed to 5 μmol PCB 126/kg mouse weight (1.63 mg/kg-day) on weeks 10, 11 and 12 (total body burden: 4.9 mg/kg). F2-Isoprostane and its metabolites, established markers of in vivo oxidative stress, measured in plasma via HPLC-MS/MS exhibited five-fold decreased levels in mice supplemented with GTE and subsequently exposed to PCB compared to animals on a control diet exposed to PCB. Livers were collected and harvested for both mRNA and protein analyses, and it was determined that many genes transcriptionally controlled by AhR and Nrf2 proteins were upregulated in PCB-exposed mice fed the green tea supplemented diet. An increased induction of genes such as SOD1, GSR, NQO1 and GST, key antioxidant enzymes, in these mice (green tea plus PCB) may explain the observed decrease in overall oxidative stress. A diet supplemented with green tea allows for an efficient antioxidant response in the presence of PCB 126 which supports the emerging paradigm that healthful nutrition may be able to bolster and buffer a physiological system against the toxicities of environmental pollutants.