Post pressure response of skin blood flowmotions in anesthetized rats with spinal cord injury
Post pressure response of skin blood flowmotions in anesthetized rats with spinal cord injury
复制标题
脊髓损伤麻醉大鼠皮肤血流运动的后压反应
DOI：
10.1016/j.mvr.2008.09.013
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发表时间：
2009-06-01
影响因子：
3.1
通讯作者：
Mak, Arthur F. T.
中科院分区：
文献类型：
--
作者：
Li, Zengyong;Tam, Eric W. C.;Mak, Arthur F. T.
压疮是脊髓损伤（SCI）患者在对身体/皮肤和底层组织施加长时间不缓解的压力时发生的常见并发症。本研究的目的是利用基于小波变换的皮肤激光多普勒血流测量（LDF）信号周期振荡的频谱分析来评估长时间压迫下麻醉脊髓损伤大鼠皮肤血流运动的充血反应。实验共选用28只Sprague-Dawley大鼠，其中正常大鼠14只，T1脊髓神经横断脊髓损伤大鼠14只。通过专门设计的压头对大鼠粗隆区施加13.3 kPa （100 mmHg）的外压。加载时间为6小时。在规定的压缩时间前后监测LDF测量20分钟。我们确定了5个频率区间（0.01-0.05 Hz、0.05-0.15 Hz、0.15-0.4 Hz、0.4-2 Hz和2-5 Hz），分别对应内皮相关代谢、神经源性、肌源性、呼吸和心脏来源。计算每个特定频率区间振荡的绝对振幅和归一化振幅，以便定量评估。比较脊髓损伤大鼠与正常大鼠的充血反应。结果表明，脊髓损伤大鼠在频率区间ⅱ（0.05 ~ 0.15 Hz）的归一化幅度明显低于正常大鼠（p . 2）
Pressure ulcer is a common complication developed in persons with spinal cord injury (SCI) when prolonged unrelieved pressure was applied to the body/skin and underlying tissues. The objective of this study is to assess the hyperemic response of the skin blood flowmotions in anesthetized rats with spinal cord injury subjected to prolonged pressure using spectral analysis based on wavelets transform of the periodic oscillations of the cutaneous laser Doppler flowmetry (LDF) signal. A total of twenty-eight Sprague-Dawley rats were used in this study, of which 14 were normal rats and the other 14 were spinal cord injured rats with transection of the T1 spinal nerves. External pressure of 13.3 kPa (100 mmHg) was applied to the trochanter area of rats via a specifically designed indentors. The loading duration was 6 h. LDF measurement was monitored for 20 min prior to and after the prescribed compression period. Five frequency intervals were identified (0.01-0.05 Hz, 0.05-0.15 Hz, 0.15-0.4 Hz, 0.4-2 Hz and 2-5 Hz) corresponding to endothelial related metabolic, neurogenic, myogenic, respiratory and cardiac origins. The absolute amplitude of oscillations of each particular frequency interval and the normalized amplitude were calculated for quantitative assessments. Comparisons of hyperemic response were performed between SCI rats and normal ones. The results showed that the normalized amplitude in the frequency interval II (0.05-0.15 Hz) was significantly lower on SCI rats than that in normal ones (p