Effect of activation with Ca ionophore A23187 and puromycin on the development of human oocytes that failed to fertilize after intracytoplasmic sperm injection
Effect of activation with Ca ionophore A23187 and puromycin on the development of human oocytes that failed to fertilize after intracytoplasmic sperm injection
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DOI：
10.1016/s0015-0282(01)01839-8
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发表时间：
2001-07-01
影响因子：
6.7
通讯作者：
Aono, T
中科院分区：
文献类型：
--
作者：
Nakagawa, K;Yamano, S;Aono, T
目的：探讨钙离子载体A23187(A23187)和嘌呤霉素序贯激活卵母细胞对卵胞浆内单精子注射(ICSI)后未受精的卵母细胞的影响。设计：实验室检查。地点：体外受精(德岛大学附属医院胚胎移植中心)。患者(S)：ICSI后18小时未发现正常受精迹象的172个卵母细胞，用A23187(5um)处理5min，再用10mug/mL的嘌霉素处理；主要结局指标(S)：计算激活率、含第二极体两个原核的卵母细胞比例(2PN2PB)和卵裂率。结果：经2PN2PB处理的卵母细胞中，有146个卵母细胞被激活(84.9%)，30.1%的卵母细胞出现2PN2PB。2PN2PB卵裂的25个卵母细胞中有16个卵裂(64.0%)。注射活动精子的卵母细胞与注射静止精子的卵母细胞的激活率、2PN2PB激活的卵母细胞比例和卵裂率均无差异。染色体分析显示，在5个可分析卵母细胞中，有4个有正常的二倍体染色体(n=46)。结论：钙离子载体A23187和嘌呤霉素序贯处理可激活ICSI后的未受精卵母细胞。含有2PN2PB的卵母细胞可以进行卵裂。(C)2001年，由美国生殖医学会提供。
Objective: To determine the effect of sequential oocyte activation with calcium ionophore A23 187 (A23187) and puromycin on oocytes that were unfertilized after intracytoplasmic sperm injection (ICSI).Design: Laboratory examination.Setting: The in vitro fertilization(embryo transfer unit in Tokushima University Hospital.Patient(s): Discarded unfertilized oocytes following ICSI.Intervention(s): All 172 oocytes that showed no evidence of normal fertilization 18 hours after ICSI were exposed to A23187 (5 muM) for 5 minutes and consequently were treated with puromycin (10 mug/mL) for; 5 hours.Main Outcome Measure(s): The activation rate, proportion of oocytes that showed two pronuclei with the second polar body (2PN2PB), and cleavage rate were calculated. Chromosomal analysis of the oocytes with 2PN2PB was also performed.Result(s): The treatment activated 146 out of 172 oocytes (84.9%) and 30.1% of the activated oocytes showed 2PN2PB. Sixteen of 25 oocytes with 2PN2PB (64.0%) cleaved. There was no difference in the activation rate, proportion of activated oocytes with 2PN2PB, or cleavage rate between oocytes that were injected; with a motile spermatozoon and those injected with an immotile spermatozoon. Chromosomal analysis showed a normal diploid set of chromosomes (n = 46) in four of five analyzable oocytes.Conclusion(s): The sequential treatment of calcium ionophore A23187 and puromycin activates unfertilized oocytes after ICSI. The resultant oocytes with 2PN2PB can cleave. (C) 2001 by American Society for Reproductive Medicine.