The ornithine decarboxylase gene of Caenorhabditis elegans: cloning, mapping and mutagenesis.
The ornithine decarboxylase gene of Caenorhabditis elegans: cloning, mapping and mutagenesis.
复制标题
秀丽隐杆线虫鸟氨酸脱羧酶基因：克隆、定位和诱变。
DOI：
10.1093/genetics/140.2.517
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发表时间：
1995
期刊：
影响因子：
3.3
通讯作者：
Coffino,P
中科院分区：
文献类型：
--
作者：
Macrae,M;Plasterk,RH;Coffino,P
The gene (odc-1) encoding ornithine decarboxylase, a key enzyme in polyamine biosynthesis, was cloned and characterized. Two introns interrupt the coding sequence of the gene. The deduced protein contains 422 amino acids and is homologous to ornithine decarboxylases of other eukaryotic species. In vitro translation of a transcript of the cDNA yielded an enzymatically active product. The mRNA is 1.5 kb in size and is formed by trans-splicing to SL1, a common 5' RNA segment. odc-1 maps to the middle of LG V, between dpy-11 and unc-42 and near a breakpoint of the nDf32 deficiency strain. Enzymatic activity is low in starved stage 1 (L1) larva and, after feeding, rises progressively as the worms develop. Targeted gene disruption was used to create a null allele. Homozygous mutants are normally viable and show no apparent defects, with the exception of a somewhat reduced brood size. In vitro assays for ornithine decarboxylase activity, however, show no detectable enzymatic activity, suggesting that ornithine decarboxylase is dispensible for nematode growth in the laboratory.
