Motor, sensory and autonomic nerve terminals containing NAP-22 immunoreactivity in the rat muscle
Motor, sensory and autonomic nerve terminals containing NAP-22 immunoreactivity in the rat muscle
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DOI：
10.1016/j.brainres.2004.01.007
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发表时间：
2004-03-26
期刊：
影响因子：
2.9
通讯作者：
Nojyo, Y
中科院分区：
文献类型：
--
作者：
Iino, S;Taguchi, K;Nojyo, Y
NAP-22是分子量为22 kDa的富含神经元的酸性蛋白，是一种Ca（2+）依赖性钙调蛋白结合蛋白，并被蛋白激酶C（PKC）磷酸化。这种蛋白质通过豆蔻酰化定位于生物膜，并在脑的膜部分和突触囊泡部分中发现。最近的研究表明，NAP-22以胆固醇依赖性方式定位于膜筏结构域，并表明NAP-22通过控制胆固醇依赖性膜动力学在神经末梢的成熟和/或维持中的作用。本研究揭示了NAP-22在大鼠肌肉周围神经中的免疫组织化学分布。在所有检查的肌肉中，运动终板中的神经末梢显示与突触囊泡和神经末梢的膜相关的NAP-22免疫反应性。在肌梭，annulospiral结束，这使得周围的梭内肌螺旋，表现出强烈的NAP-22免疫反应。肌内血管周围的自主神经纤维也显示出NAP-22的免疫反应性。从出生到成年（出生后100天），在这些周围神经中观察到NAP-22免疫反应性。虽然生长相关蛋白-43（GAP-43）的免疫反应性在这些神经中观察到从出生后，这种免疫反应性下降，从出生后20天。这些发现表明，NAP-22分布在周围神经系统的运动，感觉和自主神经末梢中并调节其功能。(C)2004 Elsevier B. V.保留所有权利。
Neuron-enriched acidic protein having a molecular mass of 22 kDa, NAP-22, is a Ca(2+)-dependent calmodulin-binding protein and is phosphorylated with protein kinase C (PKC). This protein is localized to the biological membrane via myristoylation and found in the membrane fraction of the brain and in the synaptic vesicle fraction. Recent studies showed that NAP-22 is localized in the membrane raft domain in a cholesterol-dependent manner and suggest a role for NAP-22 in maturation and/or maintenance of nerve terminals by controlling cholesterol-dependent membrane dynamics. The present study revealed the immunohistochemical distribution of NAP-22 in the peripheral nerves in rat muscles. In all examined muscles, nerve terminals in the motor endplates showed NAP-22 immunoreactivity associated with the membranes of synaptic vesicles and nerve terminals. In the muscle spindles, annulospiral endings, which made spirals around the intrafusal muscles, showed intense NAP-22 immunoreactivity. Autonomic nerve fibers around the intramuscular blood vessels also showed the immunoreactivity for NAP-22. NAP-22 immunoreactivity in these peripheral nerves was observed from birth to adulthood (100 days after birth). Though growth-associated protein-43 (GAP-43) immunoreactivity in these nerves was observed from birth, this immunoreactivity decreased from 20 days after birth. These findings suggest that NAP-22 is distributed and regulates functions in the motor, sensory and autonomic nerve terminals in the peripheral nervous system. (C) 2004 Elsevier B.V. All rights reserved.