Mastitis diagnostic tests to estimate mammary gland epithelial cell damage.
Mastitis diagnostic tests to estimate mammary gland epithelial cell damage.
复制标题
乳腺炎诊断测试可评估乳腺上皮细胞损伤。
DOI：
10.3168/jds.s0022-0302(80)83035-9
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发表时间：
1980
影响因子：
3.5
通讯作者：
M. Salmon
中科院分区：
文献类型：
--
作者：
B. Kitchen;G. Middleton;I. G. Durward;R. Andrews;M. Salmon
对乳区前乳样品、血清和乳腺组织提取物进行了N-乙酰-B-D-氨基葡萄糖、乳酸脱氢酶、谷氨酸-草酰乙酸转氨酶、芳基酯酶、牛血清白蛋白、钠、电导率和体细胞计数检测。牛血清白蛋白、芳基酯酶和钠在血清中较高，而前三种在乳腺组织中较高。随着体细胞数的增加，乳中的所有这些成分都增加。体细胞计数与各组分之间的相关系数依次为0.84、0.53、0.55、0.81、0.74和0.75。其中一些程序（例如，血清白蛋白、脱氢酶和转氨酶）用于评估乳房上皮细胞损伤程度的价值较低，因为分析时间长、样品制备繁琐和分析程序不令人满意。最适合测量乳房组织上皮损伤的方法是氨基葡萄糖苷酶试验，该试验简单快速，样品通量高。它应该是一个有用的诊断援助乳腺炎监测计划。
Quarter fore-milk samples, blood serum, and mammary gland tissue extracts were tested for N-acetyl-B-D-glucosaminidae, lactate dehydrogenase, glutamate--oxaloacetate transmaninase, arylesterase, bovine serum albumin, sodium, conductivity, and somatic cell count. Bovine serum albumin, arylesterase, and sodium were high in blood serum while the first three listed were high in mammary gland tissue. All of these components in milk increased as somatic cell count increased. Correlation coefficients between somatic cell count and each component in order were .84, .53, .55, .81, .74, and .75. Some of these procedures (e.g., serum albumin, dehydrogenase, and transaminae) for estimating the extent of udder epithelial cell damage were less valuable because of lengthy assay times, tedious sample preparation, and unsatisfactory assay procedures. The most suitable approach for measuring udder tissue epithelial damage was the glucosaminidase test which was simple and rapid with high sample throughput. It should be a useful diagnostic aid in mastitis monitoring programs.