VEGF Microsphere Technology to Enhance Vascularization in Fat Grafting
VEGF Microsphere Technology to Enhance Vascularization in Fat Grafting
复制标题
DOI：
10.1097/sap.0b013e3182573827
复制
发表时间：
2012-08-01
影响因子：
1.5
通讯作者：
Rubin, J. Peter
中科院分区：
文献类型：
--
作者：
Chung, Christopher W.;Marra, Kacey G.;Rubin, J. Peter
血管化是重建手术中工程组织植入的关键。微球中的多肽可以有效地运输到其作用部位并以持续剂量释放。我们评估了在脂肪抽吸物支架中递送血管内皮生长因子（VEGF）包封的微球对血管形成和组织存活的影响。将人脂肪抽吸物中的VEGF负载的（n = 6）和空的（n = 6）聚（乳酸-共-乙醇酸）微球和单独的人脂肪抽吸物（n = 6）皮下注射到无胸腺裸鼠的侧腹中。每组处死3只小鼠，并在第3周和第6周取出移植物。在第3周和第6周观察到VEGF样品的质量和体积增加，以及空样品和仅脂肪抽吸物样品的减少，在第6周达到统计学显著性。苏木精-伊红和CD 31（+）成像显示，在3周和6周时，VEGF样本中的血管化显著高于空组和仅脂肪抽吸物组。
Vascularization is crucial for implantation of engineered tissues in reconstructive surgery. Polypeptides encapsulated in microspheres can be efficiently transported to their site of action and released in a sustained dosage. We evaluated the effect of delivering vascular endothelial growth factor (VEGF)-encapsulated microspheres in a lipoaspirate scaffold on vascularization and tissue survival. The VEGF-loaded (n = 6) and empty (n = 6) poly(lactic-co-glycolic acid) microspheres in human lipoaspirate and the human lipoaspirate alone (n = 6) were injected subcutaneously into the flanks of athymic nude mice. Three mice from each group were killed, and grafts were explanted at weeks 3 and 6. Increases in mass and volume of VEGF samples, as well as decreases in empty and lipoaspirate-only samples, were observed at 3 and 6 weeks, reaching statistical significance at 6 weeks. Hematoxylin and eosin and CD31(+) imaging demonstrated significantly greater vascularization in VEGF samples than in both the empty and lipoaspirate-only groups at both 3 and 6 weeks.