Interaction between oxidative stress and gamma-linolenic acid in impaired neurovascular function of diabetic rats
Interaction between oxidative stress and gamma-linolenic acid in impaired neurovascular function of diabetic rats
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DOI：
10.1152/ajpendo.1996.271.3.e471
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发表时间：
1996-09-01
影响因子：
5.1
通讯作者：
Cotter, MA
中科院分区：
文献类型：
--
作者：
Cameron, NE;Cotter, MA
氧化应激与γ -亚麻酸在糖尿病大鼠神经血管功能受损中的相互作用。点。[j] .中国生物医学工程。中国生物医学工程学报，2004,25(3):471- 476。-糖尿病大鼠的神经传导和灌注缺陷依赖于氧化应激增加和n-6必需脂肪酸代谢受损，这分别通过自由基清除剂和富含γ -亚麻酸（GLA）的油治疗得到纠正。我们通过使用低剂量抗氧化剂（BM15.0639）和GLA单独或联合治疗，研究了这些机制对传导速度和神经内膜血流的相互作用。链脲佐菌素诱导糖尿病8周后，坐骨运动传导速度降低20.9%。最后2周用GLA或BM15.0639治疗，分别使这一缺陷减少18.5%和20.0%；然而，联合治疗导致71.5%的改善，对应于个体药物效应的7.5倍放大。经GLA和BM15.0639治疗后，坐骨神经营养内皮血流的48.3%缺陷分别纠正了34.8%和24.8%。联合治疗时，72.5%的血流改善大于单个药物作用的预期，表明促进相互作用。因此，联合抗氧化剂和n-6必需脂肪酸治疗的协同作用可能会增加对糖尿病神经病变的治疗能力。
Interaction between oxidative stress and gamma-linolenic acid in impaired neurovascular function of diabetic rats. Am. J. Physiol. 271 (Endocrinol. Metab. 34): E471-E476, 1996.-Nerve conduction and perfusion deficits in diabetic rats depend on increased oxidative stress and impaired n-6 essential fatty acid metabolism, which are corrected by free radical scavenger and gamma-linolenic acid (GLA)-rich oil treatments, respectively. We investigated the interaction between these mechanisms on conduction velocity and endoneurial blood flow by use of low-dose antioxidant (BM15.0639) and GLA treatments, alone and in combination. After 8 wk of streptozotocin-induced diabetes, sciatic motor conduction velocity was 20.9% reduced. Treatment with GLA or BM15.0639 for the final 2 wk corrected this deficit by 18.5 and 20.0%, respectively; however, joint treatment caused 71.5% improvement-corresponding to a 7.5-fold amplification of individual drug effects. A 48.3% deficit in sciatic nutritive endoneurial blood flow was corrected by 34.8 and 24.8% with GLA and BM15.0639 treatments, respectively. With joint treatment, the flow improvement of 72.5% was greater than expected from individual drug effects, indicating a facilitatory interaction. Thus the synergistic effect of combined antioxidant and n-6 essential fatty acid treatment-could potentially provide increased therapeutic power against diabetic neuropathy.