Quantitative immunoassay of Treponema denticola serovar C in adult periodontitis
Quantitative immunoassay of Treponema denticola serovar C in adult periodontitis
复制标题
成人牙周炎中齿螺旋体C血清型的定量免疫分析
DOI：
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发表时间：
1990
影响因子：
9.4
通讯作者：
H. Morton
中科院分区：
文献类型：
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作者：
L. Simonson;C. Goodman;H. Morton
本研究制备并鉴定了齿垢密螺旋体血清型C特异性鼠单克隆抗体。然后用这些单克隆抗体建立了免疫测定法，并对T。对牙周炎患者和健康志愿者的龈下菌斑中的齿垢菌血清型C抗原含量进行定量。人类龈下菌斑样本根据疾病的严重程度和采集部位的牙周袋深度进行分组。霸王发现严重牙周炎患者的深袋（大于6mm）中每毫克龈下菌斑的C型齿垢菌血清型含量是从中度牙周炎患者的深袋（4至6mm）中收集的样品或从健康受试者（袋深度，小于4mm）中收集的样品的两倍。
Murine monoclonal antibodies specific for Treponema denticola serovar C were produced and characterized in this study. An immunoassay was then developed by using these monoclonal antibodies, and the T. denticola serovar C antigen content of subgingival plaque was quantitated for samples taken from patients with periodontitis and healthy volunteers. The human subgingival plaque samples were grouped by severity of disease and pocket depth measurements at the collection site. The T. denticola serovar C content per milligram of subgingival plaque from deep pockets (greater than 6 mm) of patients with severe periodontitis was found to be twice that of samples collected from deep pockets (4 to 6 mm) of patients with moderate periodontitis or samples collected from healthy subjects (pocket depth, less than 4 mm).