Independent Roles of Molecular Mobility and Zeta Potential on Supramolecular Surfaces in the Sequence of RAW264.7 Macrophage Responses
Independent Roles of Molecular Mobility and Zeta Potential on Supramolecular Surfaces in the Sequence of RAW264.7 Macrophage Responses
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DOI：
10.1002/mabi.202200282
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发表时间：
2022-09-09
影响因子：
4.6
通讯作者：
Yui, Nobuhiko
中科院分区：
文献类型：
--
作者：
Tanaka-Takemura, Yuka;Arisaka, Yoshinori;Yui, Nobuhiko
生物材料的表面性质影响巨噬细胞的形态和炎症反应。最近，利用这些特性的生物材料设计已被探索用于构建用于平衡体内免疫系统的支架。在本研究中，聚轮烷表面具有不同的功能基团，包括甲基，氨基，和磺基，以澄清分子流动性和zeta电位的这些表面上的RAW 264.7巨噬细胞的反应的影响。在接种后24小时，大多数细胞粘附在每个表面上，并且初始扩散被更多带负电荷的聚轮烷表面抑制。从24至48小时的孵育，未改性和甲基化的表面上的铺展面积显着增加，而胺化和磺化表面上的铺展面积保持不变。这些结果表明，最初的细胞铺展过程取决于zeta电位，而随后的铺展过程是由分子的流动性。脂多糖刺激后，较低的移动的表面诱导更高的炎症相关基因的表达比高度移动的表面，表明分子的流动性是调节巨噬细胞的炎症活动的主要因素。这些发现表明，聚轮烷表面的zeta电位和分子流动性可能在巨噬细胞反应的顺序中发挥独立的作用。
Surface properties of biomaterials affect the morphologies and inflammatory responses of macrophages. Recently, biomaterial design utilizing these properties has been explored to build a scaffold for balancing the immune system in vivo. In the present study, polyrotaxane surfaces with different functional groups including methyl, amino, and sulfo groups are utilized to clarify the effect of molecular mobility and zeta potential of these surfaces on RAW264.7 macrophage responses. At 24 h post-seeding, the majority of the cells adhere onto each surface, and the initial spreading is suppressed by more negatively-charged polyrotaxane surfaces. From 24 to 48 h of incubation, the spreading areas on the unmodified and methylated surfaces significantly increase, whereas those on the aminated and sulfonated surfaces remain unchanged. These results suggest that the initially cellular spreading process depends on the zeta potential, while the subsequent spreading process is governed by the molecular mobility. After lipopolysaccharide stimulation, the less mobile surfaces induce higher expression of inflammation-related genes than highly mobile surfaces, suggesting that molecular mobility is the main factor modulating the inflammatory activity in macrophages. These findings indicate that the zeta potential and molecular mobility of polyrotaxane surfaces may play independent roles in the sequence of macrophage responses.