RAPID ACID-HYDROLYSIS OF PLANT-CELL WALL POLYSACCHARIDES AND SIMPLIFIED QUANTITATIVE-DETERMINATION OF THEIR NEUTRAL MONOSACCHARIDES BY GAS-LIQUID CHROMATOGRAPHY
RAPID ACID-HYDROLYSIS OF PLANT-CELL WALL POLYSACCHARIDES AND SIMPLIFIED QUANTITATIVE-DETERMINATION OF THEIR NEUTRAL MONOSACCHARIDES BY GAS-LIQUID CHROMATOGRAPHY
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DOI：
10.1021/jf00086a020
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发表时间：
1989-03-01
影响因子：
6.1
通讯作者：
DELORTLAVAL, J
中科院分区：
文献类型：
--
作者：
HOEBLER, C;BARRY, JL;DELORTLAVAL, J
本文介绍了一种快速测定植物细胞全中性多糖的方法。一个两步酸水解过程，适用于广泛的植物细胞壁材料，已经开发。研究了底物颗粒大小、反应时间、温度对不同底物（微晶纤维素、麦草、甜菜浆、大豆船体、向日葵壳）水解速率的影响。在所测试的参数中，样品粒度和初始水解温度是影响植物细胞壁多糖酸降解的主要因素。用细磨的材料（平均颗粒尺寸80 × 105）获得最大的底物水解速率。10-3 mm）在25 ℃下在72%硫酸中进行30分钟的初级水解，然后在沸腾的2N硫酸中进行120分钟的二级水解。通过水解释放的中性糖在还原和乙酰化后通过气液色谱法定量测定。在优化的方法中，糖醇乙酸酯被完全回收，并消除了干扰色谱分离的试剂。所提出的程序的有效性已被测试与各种植物材料。
A rapid method for the determination of plant cell all neutral polysaccharides is described. A two-step acid hydrolysis procedure, suitable for a wide range of plant cell wall materials, has been developed. The effect of several parametes (substrate paritcle size, reaction time, temperature) on the hydrolysis rate of various substrates (microcrystallin cellulose, wheat straw, beet pulp, soybean hull, sunflower husk) has been studied. Among tested parameters, sample particle size and primary hydrolysis temperature predominantly affect acid degradation of plant cell wall polysaccharides. Maximal substrate hydrolysis rate is obtained with finely ground materials (average particle size 80 .times. 10-3 mm) submitted to a 30-min primary hydrolysis carried out at 25.degree.C in 72% sulfuric acid, followed by a 120-min secondary hydrolysis in boiling 2 N sulfuric acid. Neutral sugars released by hydrolysis are quantitatively determined, after reduction and acetylation, by gas-liquid chromatography. In the optimized procedure, alditol acetates are totally recovered and reagents interfering in chromatographic separation are eliminated. The validity of the proposed procedure has been tested with various plant materials.