Epidemiological and virological characteristics of 2 subgroups of hepatitis B virus genotype C
Epidemiological and virological characteristics of 2 subgroups of hepatitis B virus genotype C
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DOI：
10.1086/430324
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发表时间：
2005-06-15
影响因子：
6.4
通讯作者：
Sung, JJY
中科院分区：
文献类型：
--
作者：
Chan, HLY;Tsui, SKW;Sung, JJY
背景本研究旨在探讨香港地区B肝炎病毒（HBV）C基因型亚型的特点及其与亚洲其他地区HBV C基因型的关系。对49株中国慢性B型HBV C基因型分离株的全基因组核苷酸序列与GenBank数据库中69株C基因型和12株非C基因型分离株的核苷酸序列进行比较。根据HBV全基因组异质性大于4%的情况，进行系统发育分析，以确定HBV C基因型的亚群。香港80%患者的HBV属于主要在东南亚（越南、泰国、缅甸和中国南部）发现的亚组，命名为HBV基因型“Cs”，其余20%患者的HBV属于另一亚组，主要在远东（韩国、日本和北方中国）发现，命名为HBV基因型“Ce”。总体而言，HBV基因型Cs和HBV基因型Ce之间的平均+/-核苷酸序列差异为4.2% +/-0.3%。当在香港患者中比较HBV基因型Ce和HBV基因型Ce时，HBV基因型Cs与发生基础核心启动子突变的更高趋势（80%对50%;）、核苷酸1858处C的更高患病率（95%对0%; P <0.001）和前C终止密码子突变的更低患病率（5%对50%;）相关。P = .002）。HBV基因型C可分为2个亚组-即基因型Ce和基因型Cs -具有不同的流行病学分布和病毒学特征。
Background. We aimed to investigate the characteristics of hepatitis B virus (HBV) genotype C subgroups in Hong Kong and their relationship with HBV genotype C in other parts of Asia.Methods. Full-genome nucleotide sequences of 49 HBV genotype C isolates from Chinese patients with chronic hepatitis B were compared with the sequences of 69 HBV genotype C isolates and 12 non - genotype C isolates in the GenBank database. Phylogenetic analysis was performed to define the subgroups of HBV genotype C on the basis of > 4% heterogeneity of the entire HBV genome.Results. HBV in 80% of patients in Hong Kong belonged to a subgroup predominantly found in Southeast Asia ( Vietnam, Thailand, Myanmar, and southern China) designated as HBV genotype "Cs," and HBV in the remaining 20% of patients belonged to another subgroup, predominantly found in the Far East ( Korea, Japan, and northern China), designated as HBV genotype "Ce." Overall, the mean +/- nucleotide sequence difference between HBV genotype Cs and HBV genotype Ce was 4.2% +/- 0.3%. When HBV genotype Ce and HBV genotype Ce were compared among patients in Hong Kong, HBV genotype Cs was associated with a higher tendency to develop basal core promoter mutations ( 80% vs. 50%;), a higher prevalence of C at nucleotide 1858 (95% vs. 0%; P < .001), and a lower prevalence of precore stop codon mutations (5% vs. 50%;. P = .002).Conclusions. HBV genotype C can be differentiated into 2 subgroups - namely, genotype Ce and genotype Cs - that have different epidemiological distributions and virological characteristics.