Immunohistochemical study of microscopic globular bodies of normal human brain.
Immunohistochemical study of microscopic globular bodies of normal human brain.
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正常人脑微观球体的免疫组织化学研究。
DOI：
10.2220/biomedres.32.337
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
K. Wakabayashi
中科院分区：
文献类型：
--
作者：
S. Odagiri;F. Mori;K. Tanji;Naohito Kuroda;K. Wakabayashi
微观球状体（MGBs）是明亮且均匀的嗜酸性球形包裹体，直径为1-10µm。它们主要分布在1 - 10岁的正常人的大脑新皮层和海马中。在超微结构上，MGBs由电子致密的粒状材料组成，位于枝晶中。然而，mgb的免疫组织化学特征是不确定的。因此，我们用免疫组织化学方法检查了5名年龄在25岁到76岁之间的对照受试者的海马体。MGBs边缘部分溶酶体蛋白酶（组织蛋白酶B、D和L）、树突（MAP2）和树突棘（drebrin）标记物呈阳性。在组织蛋白中，mgb最常被抗组织蛋白d免疫标记。它们的泛素、泛素-蛋白酶体系统（p62、NUB1和EDD1）、自噬体（LC3）、细胞骨架蛋白（神经丝蛋白、肌动蛋白、微管蛋白和细胞角蛋白）、tau蛋白、α-突触核蛋白和TDP-43均阴性。这些发现表明mgb是通过溶酶体-蛋白酶系统而不是泛素-蛋白酶体系统或自噬体进行隔离的。
Microscopic globular bodies (MGBs) are brilliantly and homogenously eosinophilic spherical inclusions, 1-10 µm in diameter. They are mainly distributed in the cerebral neocortex and hippocampus in normal individuals ranged in age from first to tenth decade. Ultrastructurally, MGBs are composed of electron-dense granular material and are located in dendrites. However, immunohistochemical profile of MGBs is uncertain. Therefore, we immunohistochemically examined the hippocampus from five control subjects ranged from 25 to 76 years. The marginal portion of MGBs was positive for lysosomal proteases (cathepsins B, D and L), and markers of dendrite (MAP2) and dendritic spine (drebrin). In some cases, MGBs were entirely immunostained with anti-cathepsin D. Among the cathepsins, MGBs were most frequently immunolabeled with anticathepsin D. They were negative for ubiquitin, ubiquitin-proteasome system (p62, NUB1 and EDD1), autophagosome (LC3), cytoskeletal proteins (neurofilament, actin, tubulin and cytokeratin), tau, α-synuclein and TDP-43. These findings suggest that MGBs are sequestered by lysosome- protease system, but not by ubiquitin-proteasome system or autophagosome.
溶酶体半胱氨酸蛋白酶组织蛋白酶 S 在阿尔茨海默病和唐氏综合症患者的大脑中增加。
DOI：
--
发表时间：
1995
期刊：
The American journal of pathology.
影响因子：
--
作者：
Lemere,CA;Munger,JS;Shi,GP;Natkin,L;Haass,C;Chapman,HA;Selkoe,DJ
通讯作者：
Selkoe,DJ