Characteristics of synovial fluid effusion in collagen-induced arthritis (CIA) in the DA rat; A comparison of histology and antibody reactivities in an experimental chronic arthritis model and rheumatoid arthritis (RA)
Characteristics of synovial fluid effusion in collagen-induced arthritis (CIA) in the DA rat; A comparison of histology and antibody reactivities in an experimental chronic arthritis model and rheumatoid arthritis (RA)
复制标题
DOI：
10.1046/j.1365-2249.1997.3311221.x
复制
发表时间：
1997-03-01
影响因子：
4.6
通讯作者：
Klareskog, L
中科院分区：
文献类型：
--
作者：
Harris, HE;Liljestrom, M;Klareskog, L
我们的特点是细胞的内容物和一些抗体反应性，从DA大鼠与CIA的滑液（SF）。由于CIA被广泛用作RA的模型，其中许多关于免疫反应的研究都是在SF样本上进行的，因此我们认为描述CIA中的局部致病免疫反应很重要。在疾病高峰期（免疫后22天），CIA SF中的主要细胞群是粒细胞（72%），但也存在巨噬细胞（17.9%）、浆细胞（2.6%）和淋巴细胞（7.7%）。同时获得的CIA滑膜（SM）主要由单核细胞浸润，也存在粒细胞、淋巴细胞和浆细胞。关节炎和正常DA大鼠血液中的细胞群没有差异。同样，高抗胶原II型（CII）和类风湿因子（RF）水平可以检测到SF和血清。值得注意的是，RF水平在正常DA大鼠中也增加。中等水平的抗热休克蛋白65 kD（热休克蛋白）抗体记录在正常和患病动物全身。总之，SF和SM中的细胞组成在大鼠CIA和RA中相似。SF和SM之间的形态学差异是RA的特征，也可以在CIA中得到证明。抗体数据表明抗CII和抗hsp抗体以及RF的全身性产生，但它们不支持关节中局部产生这些抗体，这是RA的情况。
We have characterized the cellular content and some antibody reactivities in synovial fluid (SF) from DA rats with CIA. Since CIA is widely used as a model for RA, in which many studies concerning immune responses are performed on SF samples, we considered it important to describe the local, disease-causing immune reactions in CIA. At the peak of disease (day 22 after immunization), the major cell population in CIA SF was granulocytes (72%), but macrophages (17.9%), plasma cells (2.6%) and lymphocytes (7.7%) were also present. The CIA synovial membrane (SM) obtained at the same time was mainly infiltrated by monocytes, with granulocytes, lymphocytes and plasma cells also present. Cell populations in blood did not differ between arthritic and normal DA rats. Equally, high anti-collagen type II (CII) and rheumatoid factor (RF) levels could be detected both in SF and in sera. Notably, RF levels were also increased in normal DA rats. Moderate levels of anti-heat shock protein 65 kD (hsp) antibodies were recorded systemically in both normal and diseased animals. In conclusion, the cellular composition in SF and in SM are similar in rat CIA and in RA. The morphological differences between SF and SM that are characteristic for RA could also be demonstrated in CIA. The antibody data indicate systemic production of anti-CII and anti-hsp antibodies as well as RF, but they give no support for local production of these antibodies in the joints, which is the case in RA.