Rice OsDOF15 contributes to ethylene-inhibited primary root elongation under salt stress
Rice OsDOF15 contributes to ethylene-inhibited primary root elongation under salt stress
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水稻 OsDOF15 有助于盐胁迫下乙烯抑制的初生根伸长
DOI：
10.1111/nph.15824
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发表时间：
2019-07-01
期刊：
影响因子：
9.4
通讯作者：
Huang, Rongfeng
中科院分区：
文献类型：
--
作者：
Qin, Hua;Wang, Juan;Huang, Rongfeng
在幼苗早期，主根通过内源激素水平的调节迅速适应环境变化。植物激素乙烯抑制初生根伸长，但环境变化如何调节乙烯减少根生长的潜在分子机制仍然知之甚少。在这里，我们发现一种新的水稻DOF转录因子OsDOF15通过限制乙烯生物合成，通过调节根分生组织中的细胞增殖，积极调节初生根伸长。OsDOF15的功能缺失会损害根的伸长和根分生组织的细胞增殖，而OsDOF15的过表达则会增强这些过程，这表明OsDOF15是根伸长的关键调节因子。这种调控涉及OsDOF15与OsACS1启动子的直接相互作用，导致乙烯生物合成的抑制。OsDOF15对乙烯生物合成的控制反过来调节根分生组织中的细胞增殖。盐胁迫抑制OsDOF15转录，OsDOF15介导的乙烯生物合成在盐胁迫下抑制初生根伸长中起作用。因此，我们的数据揭示了乙烯抑制主根伸长是如何受到OsDOF15对环境信号的精细控制的，这是植物响应盐胁迫并将信息传递给乙烯生物合成以限制根伸长的新机制。
In early seedlings, the primary root adapts rapidly to environmental changes through the modulation of endogenous hormone levels. The phytohormone ethylene inhibits primary root elongation, but the underlying molecular mechanism of how ethylene-reduced root growth is modulated in environmental changes remains poorly understood. Here, we show that a novel rice (Oryza sativa) DOF transcription factor OsDOF15 positively regulates primary root elongation by regulating cell proliferation in the root meristem, via restricting ethylene biosynthesis. Loss-of-function of OsDOF15 impaired primary root elongation and cell proliferation in the root meristem, whereas OsDOF15 overexpression enhanced these processes, indicating that OsDOF15 is a key regulator of primary root elongation. This regulation involves the direct interaction of OsDOF15 with the promoter of OsACS1, resulting in the repression of ethylene biosynthesis. The control of ethylene biosynthesis by OsDOF15 in turn regulates cell proliferation in the root meristem. OsDOF15 transcription is repressed by salt stress, and OsDOF15-mediated ethylene biosynthesis plays a role in inhibition of primary root elongation by salt stress. Thus, our data reveal how the ethylene-inhibited primary root elongation is finely controlled by OsDOF15 in response to environmental signal, a novel mechanism of plants responding to salt stress and transmitting the information to ethylene biosynthesis to restrict root elongation.