The effect of low intensity pulsed ultrasound applied to rabbit tibiae during the consolidation phase of distraction osteogenesis
The effect of low intensity pulsed ultrasound applied to rabbit tibiae during the consolidation phase of distraction osteogenesis
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DOI：
10.1016/s0736-0266(02)00003-7
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发表时间：
2002-07
影响因子：
2.8
通讯作者：
J. Tis;R. Meffert;N. Inoue;E. McCarthy;M. Shaun Machen;K. Mchale;E. Chao
中科院分区：
文献类型：
--
作者：
J. Tis;R. Meffert;N. Inoue;E. McCarthy;M. Shaun Machen;K. Mchale;E. Chao
本研究的目的是确定低强度脉冲超声（LIPU）是否加速兔模型牵张后再生骨的成熟。对26只新西兰白色家兔进行胫骨中段截骨术，并在前内侧应用外固定器。在7天的潜伏期后，每12小时牵开胫骨0.5mm，持续10天。从第17天至第37天处死，13只家兔接受LIPU 20 min/天（治疗组），13只接受假LIPU（对照组）。牵引后每周拍摄X线片，测量骨痂的总面积和矿化面积。处死后，进行双能X线吸收测定、扭转失效试验和组织形态测定。超声处理的胫骨硬度平均为对侧胫骨的68.8 ± 3.8%，强度平均为对侧胫骨的68.2 ± 6.0%。对照侧胫骨的刚度为对侧胫骨的78.7 ± 7.0%，强度为对侧胫骨的70.2 ± 7.9%。刚度和强度的差异不显著（分别为p= 0.39和0.81），与研究中试验的动物数量有关。治疗组的密度为对侧的91.6%，对照组为对侧的88.5%（p= 0.84）。X线片显示，与对照组胫骨相比，牵引后1、2和3周，LIPU处理胫骨中的骨痂显著更大（分别为p< 0.01、0.008和0.05）。组织形态测定显示，与对照组相比，LIPU处理的胫骨中纤维组织显著减少（p< 0.05），并且LIPU处理的胫骨中有更强的骨趋势（p= 0.06）。发现LIPU增加了牵张骨痂的大小，并且可能改变再生骨的组成，但在牵张成骨的巩固阶段应用时，它对再生骨的机械性能或密度没有积极影响。由Elsevier Science Ltd.代表骨科研究学会出版。© 2002骨科研究学会。由爱思唯尔科技有限公司出版。保留所有权利。
The purpose of this study was to determine if low intensity pulsed ultrasound (LIPU) accelerated the maturation of regenerate bone when applied after distraction in a rabbit model. A mid‐tibial osteotomy was performed in 26 New Zealand white rabbits and an external fixator applied anteromedially. After a seven day latency period, the tibiae were distracted 0.5 mm every 12 h for 10 days. Thirteen of the rabbits received LIPU for 20 min/day (treatment group) and 13 received sham LIPU (control group) from day 17 until sacrifice on day 37. Radiographs were taken weekly after distraction and the total and mineralized areas of the callus were measured. After sacrifice, dual‐energy X‐ray absorptiometry, torsional testing to failure, and histomorphometry were performed. Ultrasound‐treated tibiae were a mean of 68.8 ± 3.8% as stiff as and 68.2 ± 6.0% as strong as the contralateral tibiae. Control tibiae were 78.7 ± 7.0% as stiff as and 70.2 ± 7.9% as strong as the contralateral tibiae. The differences in stiffness and strength were not significant (p= 0.39 and 0.81, respectively) with the number of the animals tested in the study. The treatment group was 91.6% as dense as the contralateral side and the control group was 88.5% as dense as the contralateral side (p= 0.84). Radiographs revealed a significantly larger callus in the LIPU‐treated tibiae at 1, 2 and 3 weeks after distraction compared to control tibiae (p< 0.01, 0.008 and 0.05, respectively). Histomorphometry revealed significantly less fibrous tissue in the LIPU‐treated tibiae (p< 0.05) and a strong trend towards more bone in the LIPU‐treated tibiae compared to controls (p= 0.06). LIPU was found to increase the size of the distraction callus and it might alter the composition of regenerate bone but it did not have a positive effect on the mechanical properties or density of regenerate bone when applied during the consolidation phase of distraction osteogenesis. Published by Elsevier Science Ltd. on behalf of Orthopaedic Research Society. © 2002 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.