Fate of bone marrow-derived cultured mast cells after intracutaneous, intraperitoneal, and intravenous transfer into genetically mast cell-deficient W/Wv mice. Evidence that cultured mast cells can give rise to both connective tissue type and mucosal mast cells.
Fate of bone marrow-derived cultured mast cells after intracutaneous, intraperitoneal, and intravenous transfer into genetically mast cell-deficient W/Wv mice. Evidence that cultured mast cells can give rise to both connective tissue type and mucosal mast cells.
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DOI：
10.1084/jem.162.3.1025
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发表时间：
1985-09-01
期刊：
影响因子：
--
通讯作者：
Galli SJ
中科院分区：
文献类型：
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作者：
Nakano T;Sonoda T;Hayashi C;Yamatodani A;Kanayama Y;Yamamura T;Asai H;Yonezawa T;Kitamura Y;Galli SJ
结缔组织肥大细胞和肥大细胞体外生长的多潜能造血干细胞，但这两个肥大细胞群体表现出许多不同的形态，生物化学和功能。我们研究了培养的肥大细胞或其后代的表型是否改变时，细胞转移到体内不同的位置。培养的肥大细胞未成熟的超微结构，并与阿辛蓝染色，但既没有番红或硫酸小檗碱，荧光染料结合到结缔组织肥大细胞颗粒的肝素。相比之下，在腹腔内注射用番红和硫酸小檗碱染色的培养的WBB6F1-+/+或C57 BL/6-bgJ/bgJ肥大细胞后10周，从先天性肥大细胞缺陷（WB X C57 BL/6）F1-W/Wv（WBB6F1-W/Wv）小鼠的腹腔中回收肥大细胞。硫酸黄连素染色被肝素酶处理的细胞，但不软骨素酶ABC，这表明过继转移的肥大细胞群已获得合成和储存肝素的能力。此外，回收的肥大细胞与WBB6F1-+/+小鼠的正常成熟腹膜肥大细胞的超微结构无法区分，并且含有比直接从培养物研究的肥大细胞更多的组胺。静脉注射培养的肥大细胞导致番红和硫酸小檗碱阳性肥大细胞在腹腔，脾脏，皮肤和腺胃固有肌层的发展。肥大细胞也在腺胃粘膜上发育，但这些细胞用阿辛蓝而不是番红染色，并且不与硫酸小檗碱染色。这一结果表明，培养的肥大细胞可以产生肥大细胞的结缔组织型或粘膜表型，这取决于解剖位置。此外，将培养的肥大细胞移植到WBB6F1-W/Wv小鼠中对受体小鼠的贫血没有可测量的影响，这表明了修复WBB6F1-W/Wv小鼠的肥大细胞缺乏而不影响其他骨髓来源的群体如红细胞的可能策略。静脉注射代表性结缔组织型肥大细胞（30 - 50%纯的源自WBB6F1-+/+小鼠的腹膜肥大细胞）得到的结果与用培养的肥大细胞获得的结果相似：用番红和硫酸黄连素染色的WBB6F1-W/Wv受体的腹腔、皮肤、脾脏和腺胃固有肌层中发育的肥大细胞，而腺胃粘膜中发育的肥大细胞仅被阿辛蓝染色。（400字处截断摘要）
Both connective tissue mast cells and mast cells grown in vitro are derived from multipotential hematopoietic stem cells, but these two mast cell populations exhibit many differences in morphology, biochemistry, and function. We investigated whether the phenotype of cultured mast cells or their progeny was altered when the cells were transferred into different locations in vivo. Cultured mast cells were immature by ultrastructure, and stained with alcian blue but with neither safranin or berberine sulfate, a fluorescent dye that binds to the heparin of connective tissue mast cell granules. By contrast, mast cells recovered from the peritoneal cavity of congenitally mast cell- deficient (WB X C57BL/6)F1-W/Wv (WBB6F1-W/Wv) mice 10 wk after intraperitoneal injection of cultured WBB6F1-+/+ or C57BL/6-bgJ/bgJ mast cells stained with both safranin and berberine sulfate. Staining with berberine sulfate was prevented by treatment of the cells with heparinase but not chondroitinase ABC, suggesting that the adoptively transferred mast cell population had acquired the ability to synthesize and store heparin. Furthermore, the recovered mast cells were indistinguishable by ultrastructure from the normal mature peritoneal mast cells of WBB6F1-+/+ mice, and contained substantially more histamine than mast cells studied directly from culture. Intravenous injection of cultured mast cells resulted in the development of safranin-and berberine sulfate-positive mast cells in the peritoneal cavity, spleen, skin, and glandular stomach muscularis propria. Mast cells also developed on the glandular stomach mucosa, but these cells stained with alcian blue rather than safranin, and did not stain with berberine sulfate. This result suggests that cultured mast cells can give rise to mast cells of either the connective tissue type or mucosal phenotype, depending on anatomical location. Furthermore, transplantation of cultured mast cells into WBB6F1-W/Wv mice had no measurable effect on the anemia of the recipient mice, suggesting a possible strategy for repairing the mast cell deficiency of WBB6F1-W/Wv mice without affecting other bone marrow-derived populations such as erythrocytes. Intravenous injection of representative connective tissue type mast cells (30-50% pure peritoneal mast cells derived from WBB6F1- +/+ mice) gave results similar to those obtained with cultured mast cells: mast cells developing in the peritoneal cavity, skin, spleen, and glandular stomach muscularis propria of WBB6F1-W/Wv recipients stained with safranin and berberine sulfate, whereas mast cells developing in the mucosa of the glandular stomach stained only with alcian blue.(ABSTRACT TRUNCATED AT 400 WORDS)