The genome sequence of a new strain of Mycobacterium ulcerans ecovar Liflandii, emerging as a sturgeon pathogen
The genome sequence of a new strain of Mycobacterium ulcerans ecovar Liflandii, emerging as a sturgeon pathogen
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作为鲟鱼病原体出现的溃疡分枝杆菌新菌株 Liflandii 的基因组序列
DOI：
10.1016/j.aquaculture.2018.02.001
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发表时间：
2018
期刊：
影响因子：
4.5
通讯作者：
["Shuhuan Zhang
中科院分区：
文献类型：
--
作者：
["Shuhuan Zhang
溃疡分枝杆菌（Mycobacteriumulcancovar Liflandii，MuLiflandii）是两栖类动物的一种非分枝杆菌病原菌。本文首次报道了一种新的MuLiflandii感染中华鲟的情况。所有病鱼均表现出腹水和/或肌肉溃疡的典型临床症状。从病鱼腹水中分离到一株生长缓慢的抗酸芽孢杆菌ASM 001，经杂交鲟试验证明该菌株具有致病性。MuLiflandii ASM 001基因组全序列为环状染色体，全长6,167,296 bp，G + C含量为65.57%，包含4518个预测编码DNA序列和999个假基因、3个rRNA操纵子和47个转运RNA序列。此外，我们在整个MuLiflandii ASM 001基因组中发现了245个IS 2404拷贝，34个微卫星和36个CRISPR序列。在MuLiflandii ASM 001的预测基因中，我们发现了临床MuLiflandii 128 FXT的203个毒力因子的直系同源物，这些毒力因子在宿主细胞侵袭、调节吞噬细胞功能和在巨噬细胞内存活中起作用。这些候选毒力因子为在分子水平上了解其致病机制提供了关键基础。利用完整的现有基因组进行比较分析表明，MuLiflandii ASM 001与MuLiflandii 128 FXT具有高度的共线性。我们预计完整的MuLiflandii ASM 001基因组序列的可用性将为MuLiflandii分离株的比较基因组研究提供宝贵的资源，并为分枝杆菌属的宿主、生态和功能多样性提供新的见解。
Mycobacteriumulceransecovar Liflandii (MuLiflandii) is emerging as a non-mycobacterial pathogen in amphibians. Here, we make the first report on the prevalence of a new strain ofMuLiflandii infection in Chinese sturgeon. All the diseased fish showed the classic clinical symptoms of ascites and/or muscle ulceration. A new slow-growing and acid-fast bacillus ASM001 strain was obtained from the ascites of infected fish; this strain demonstrated pathogenicity when tested in hybrid sturgeon. The complete genome sequence ofMuLiflandii ASM001 is a circular chromosome of 6,167,296 bp, with a G + C content of 65.57%, containing 4518 predicted coding DNA sequences and 999 pseudo-genes, 3 rRNA operons, and 47 transfer RNA sequences. In addition, we found 245 copies of IS2404, 34 microsatellites, and 36 CRISPR sequences in the wholeMuLiflandii ASM001 genome. Among the predicted genes ofMuLiflandii ASM001, we found orthologs of 203 virulence factors of clinicalMuLiflandii 128FXT operating in host cell invasion, modulation of phagocyte function, and survival inside the macrophages. These virulence factor candidates provide a key basis for understanding their pathogenic mechanisms at the molecular level. A comparative analysis that used complete, existing genomes showed thatMuLiflandii ASM001 has high synteny withMuLiflandii 128FXT. We anticipate the availability of the completeMuLiflandii ASM001 genome sequence will provide a valuable resource for comparative genomic studies ofMuLiflandii isolates, as well as provide new insights into the host, ecological, and functional diversity of the genusMycobacterium.
非洲热带爪蛙（热带爪蟾）群体中溃疡分枝杆菌样感染的特征。
DOI：
--
发表时间：
2004
期刊：
Comparative medicine.
影响因子：
--
作者：
Trott,KristinA;Stacy,BrianA;Lifland,BarryD;Diggs,HelenE;Harland,RichardM;Khokha,MustafaK;Grammer,TimothyC;Parker,JohnM
通讯作者：
Parker,JohnM
影响因子：
14.9
作者：
Salzberg, SL;Delcher, AL;White, O
通讯作者：
White, O
影响因子：
7
作者：
Darling, ACE;Mau, B;Perna, NT
通讯作者：
Perna, NT