Guinea pig infected with Mycobacterium tuberculosis via oral consumption
Guinea pig infected with Mycobacterium tuberculosis via oral consumption
复制标题
豚鼠经口感染结核杆菌
DOI：
10.1080/09712119.2018.1505622
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发表时间：
2018-07-31
影响因子：
1.4
通讯作者：
Ho, Wenzhe
中科院分区：
文献类型：
--
作者：
Gao, Jianfeng;Guo, Ming;Ho, Wenzhe
摘要豚鼠是研究结核分枝杆菌（Mtb）感染的非常合适的动物模型，因为它与人类的Mtb肺部感染具有相似性。已知豚鼠可通过呼吸道或皮下暴露于Mtb而被有效感染。然而，文献中几乎没有关于经口感染Mtb的信息。在这里，我们研究了豚鼠是否可以通过饮用含有结核分枝杆菌的水而感染。我们的研究结果证实豚鼠可以通过饮用有毒水感染Mtb。感染后第30天，豚鼠出现均匀的椭圆形火山口样溃疡。细菌培养显示，在肺和脾中的Mtb生长从感染高剂量的Mtb的豚鼠。此外，感染动物的肺、脾和肠系膜淋巴结中存在组织病理学肉芽肿性病变。我们提供了令人信服的证据表明，豚鼠可以通过饮用含有结核分枝杆菌的水而感染。感染动物的临床和病理学观察结果与通过呼吸道或皮下途径感染的豚鼠相似。
ABSTRACT Guinea pig represents a highly suitable animal model for study of Mycobacterium tuberculosis(Mtb)infection, as it demonstrates similarities to Mtb pulmonary infection in humans. It is known that guinea pigs can be efficiently infected by the respiratory or subcutaneous exposure to Mtb. However, information on Mtb infection through oral route is almost absent in the literature. Here, we examined whether guinea pigs can be infected by drinking Mtb containing water. Our findings confirmed that the guinea pigs could be infected with Mtb via drinking virulent water. The infected guinea pigs developed uniform oval-craterform ulcers at the 30th day after infection. Bacterial cultures showed Mtb growth in the lungs and spleens from the guinea pigs infected with high dose of Mtb. In addition, the infected animals had histopathological granulomatous lesions in lungs, spleens and mesenteric lymph nodes. We provide the compelling evidence that the guinea pigs could be infected by drinking Mtb containing water. The clinical and pathological observations in the infected animals were similar to those found in guinea pigs infected via the respiratory or subcutaneous routes.