The histaminergic innervation of the lateral geniculate complex in the cat.
The histaminergic innervation of the lateral geniculate complex in the cat.
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猫外侧膝状体复合体的组胺能神经支配。
DOI：
10.1017/s0952523800003631
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发表时间：
1993
影响因子：
1.9
通讯作者：
Pienkowski,TP
中科院分区：
文献类型：
--
作者：
Uhlrich,DJ;Manning,KA;Pienkowski,TP
本文用组胺抗体对猫丘脑背侧、腹侧外侧膝状体核和膝周核的组胺能神经支配进行了免疫组织化学研究，发现猫脑内组胺免疫反应阳性神经元主要集中在下丘脑后部的腹外侧部，证实了以前的报道。然而，这个细胞群也蔓延到内侧，背侧，和极外侧区域的后下丘脑和延伸远喙视交叉。组胺标记的纤维覆盖所有区域的外侧膝状体复合体，但标记的密度变化。腹侧外侧膝状体核（vLGN）是最密集的标记，背侧外侧膝状体的A层稀疏标记，膝状体C层和周膝状体核显示中间量的标签。因此，组胺能纤维表现出对W细胞系统支配的区域的偏好。标记的纤维表现出很少的分支和许多的直通的分支，使其呈现珠状外观。在vLGN中，标记纤维的直径较大（最大可达2 μm），下丘脑注射生物素标记物后，在外侧膝状体复合体中发现了标记纤维。顺行标记纤维在形态、分布和相对终扣大小上与组胺能纤维相似。因此，下丘脑似乎是来源的组胺能纤维在外侧膝状体复合体。组胺标记的纤维在背外侧膝状体核（dLGN）表现出罕见的超微结构形态。许多非常大的、圆形或椭圆形的小泡充满纤维囊。突触直接与其他各种树突和轴突轮廓并列，但迄今为止还没有令人信服的突触接触。这些组胺能纤维的分布和外观类似于那些报道的组胺能纤维。我们的研究结果支持这样的想法，即组胺工程nonsynaptically作为一种神经调质在外侧膝状体复合体，影响视觉唤醒的水平。
The histaminergic innervation of the thalamic dorsal and ventral lateral geniculate nuclei and the perigeniculate nucleus of the cat was examined immunohistochemically by means of an antibody to histamine.We find histamine-immunoreactive neurons in the cat brain are concentrated in the ventrolateral portion of the posterior hypothalamus, confirming a previous report. However, this cell group also spreads into medial, dorsal, and extreme lateral regions of the posterior hypothalamus and extends as far rostral as the optic chiasm.Histamine-labeled fibers cover all regions of the lateral geniculate complex, but the density of labeling varies. The ventral lateral geniculate nucleus (vLGN) is most densely labeled, the A laminae of the dorsal lateral geniculate are sparsely labeled, and the geniculate C laminae and the perigeniculate nucleus show intermediate amounts of label. Thus, histaminergic fibers demonstrate a predilection for zones innervated by the W-cell system. Labeled fibers exhibit few branchings and numerous en passant swellings, lending a beaded appearance. The vLGN showed more instances of fibers with larger-sized swellings (up to 2 μm).Following injections of biotinylated tracers into the hypothalamus, we find labeled fibers throughout the lateral geniculate complex. The anterogradely labeled fibers resemblethe histaminergic fibers in morphology, distribution, and relative bouton size. Thus, the hypothalamus appears to be the source of the histaminergic fibers in the lateral geniculate complex.Histamine-labeled fibers in the dorsal lateral geniculate nucleus (dLGN) exhibit uncommon ultrastructural morphology. Many extremely large, round, or elliptical vesicles fill the fiber swellings. Swellings are directly apposed to a variety of other dendritic and axonal profiles, but thus far no convincing synaptic contacts have been seen. The distribution and appearance of these histaminergic fibers resembles those reported for serotonergic fibers.Our results support the idea that histamine works nonsynaptically as a neuromodulator in the lateral geniculate complex, affecting the level of visual arousal.