3-DIMENSIONAL GROWTH AND DIFFERENTIATION OF OVARIAN TUMOR-CELL LINE IN HIGH ASPECT ROTATING-WALL VESSEL - MORPHOLOGIC AND EMBRYOLOGIC CONSIDERATIONS
3-DIMENSIONAL GROWTH AND DIFFERENTIATION OF OVARIAN TUMOR-CELL LINE IN HIGH ASPECT ROTATING-WALL VESSEL - MORPHOLOGIC AND EMBRYOLOGIC CONSIDERATIONS
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DOI：
10.1002/jcb.240510307
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发表时间：
1993-03-01
影响因子：
4
通讯作者：
GOODWIN, TJ
中科院分区：
文献类型：
--
作者：
BECKER, JL;PREWETT, TL;GOODWIN, TJ
卵巢癌是妇科恶性肿瘤死亡的主要原因。为了更好地了解这些侵袭性肿瘤，开发研究人类卵巢癌的体外模型至关重要。然而，由于在原代培养中生长的患者肿瘤细胞的普遍较差的存活率，长期细胞系的建立一直是困难的。令人满意的培养系统卵巢肿瘤细胞因此受到限制。为了研究这些肿瘤的生长和分化中涉及的细胞相互作用，从卵巢混合苗勒管肿瘤建立细胞系。将该细胞系命名为LN 1，在高纵横比侧壁容器中的微载体珠上培养。在该血管中生长的肿瘤细胞容易增殖，而不需要与支持细胞层共培养。通过相差光学显微镜和扫描电子显微镜对细胞形态进行评价，发现存在由桥接在一起的多个细胞包被的珠粒组成的三维多细胞聚集体，以及分散的LN 1细胞聚集体，其作为悬浮液中游离的球状体增殖。与传统的培养系统相比，在高纵横比侧壁容器中的培养促进了可以回收的多种细胞类型的产生。这些结果说明了该培养系统提供多能细胞生长所必需的生物学条件的能力。
Cancer of the ovary is the leading cause of death from gynecologic malignancy. To understand better these aggressive tumors, the development of in vitro models to study human ovarian cancer is critical. However, the establishment of long-term cell lines has been difficult, due to the generalized poor survival of patient tumor cells grown in primary culture. Satisfactory culture systems for ovarian tumor cells have therefore been limited. To study cellular interactions involved in the growth and differentiation of these tumors, a cell line was established from a mixed mullerian tumor of the ovary. This cell line, designated LN1, was cultured on microcarrier beads in the high aspect rotating-wall vessel. The tumor cells grown in this vessel readily proliferated without a requirement for cocultivation with a supportive cell layer. Evaluation of cellular morphology by phase contrast light microscopy and scanning electron microscopy revealed the presence of three-dimensional multicellular aggregates consisting of multiple cell-coated beads bridged together, as well as scattered aggregates of LN1 cells proliferating as spheroids free in suspension. In contrast to conventional culture systems, culture in the high aspect rotating-wall vessel facilitated the generation of multiple cell types that could be recovered. These results illustrate the ability of this culture system to provide the biological conditions necessary for pluripotent cell growth.