A plant viral coat protein RNA binding consensus sequence contains a crucial arginine
A plant viral coat protein RNA binding consensus sequence contains a crucial arginine
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DOI：
10.1002/j.1460-2075.1996.tb00888.x
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发表时间：
1996-09-16
期刊：
影响因子：
11.4
通讯作者：
Gehrke, L
中科院分区：
文献类型：
--
作者：
AnselMcKinney, P;Scott, SW;Gehrke, L
紫花苜蓿花叶病毒(AMV)和伊拉尔病毒[型病毒：烟草条纹病毒(TSV)]的一个显著特征是，除了基因组RNA外，病毒外壳蛋白还需要病毒外壳蛋白来建立对植物的侵染，AMV和TSV外壳蛋白在RNA结合和侵染起始方面几乎没有共同的氨基酸序列，但在功能上是可以互换的。AMV外壳蛋白富含赖氨酸的氨基末端RNA结合域缺乏先前发现的RNA结合基序。这里，AMV外壳蛋白RNA结合域包含一个精氨酸，其特定的侧链和位置对RNA结合至关重要。此外，还鉴定了两个IarVirus外壳蛋白TSV和柑橘杂色病毒的假定RNA结合域，并发现其含有一个关键的精氨酸。AMV和IarVirus外壳蛋白序列的比对发现了一个以精氨酸为中心的新的RNA结合共识序列，这一共识可能部分解释了为什么异源病毒RNA-外壳蛋白混合物具有传染性。
A defining feature of alfalfa mosaic virus (AMV) and ilarviruses [type virus: tobacco streak virus (TSV)] is that, in addition to genomic RNAs, viral coat protein is required to establish infection in plants, AMV and TSV coat proteins, which share little primary amino acid sequence identity, are functionally interchangeable in RNA binding and initiation of infection. The lysine-rich amino-terminal RNA binding domain of the AMV coat protein lacks previously identified RNA binding motifs. Here, the AMV coat protein RNA binding domain is shown to contain a single arginine whose specific side chain and position are crucial for RNA binding, In addition, the putative RNA binding domain of two ilarvirus coat proteins, TSV and citrus variegation virus, is identified and also shown to contain a crucial arginine. AMV and ilarvirus coat protein sequence alignment centering on the key arginine revealed a new RNA binding consensus sequence, This consensus may explain in part why heterologous viral RNA-coat protein mixtures are infectious.