Neurokinin B gene expression is increased in the arcuate nucleus of ovariectomized rats.
Neurokinin B gene expression is increased in the arcuate nucleus of ovariectomized rats.
复制标题
去卵巢大鼠的弓状核中神经激肽 B 基因表达增加。
DOI：
10.1159/000126768
复制
发表时间：
1994
影响因子：
4.1
通讯作者：
Bruce,TR
中科院分区：
文献类型：
--
作者：
Rance,NE;Bruce,TR
绝经后妇女漏斗核（弓状核）速激肽神经元肥大和基因表达增加。我们假设绝经后妇女下丘脑速激肽基因表达的改变是继发于卵巢功能衰竭，而不是由于年龄本身。在这项研究中，原位杂交和计算机辅助显微镜，以确定是否卵巢切除术调节神经激肽B（NKB），P物质（SP）或前阿黑皮素（POMC）基因在大鼠弓状核的表达。检查了四组：发情前期;发情间期第1天;卵巢切除，并通过单次注射20 mg/kg戊酸雌二醇诱导恒定发情。处理后2个月处死大鼠。计算机辅助显微镜用于确定速激肽神经元的数量、细胞面积和标记神经元的放射自显影颗粒密度。我们报告了卵巢切除大鼠NKB基因表达的显著变化。去卵巢大鼠含NKB基因转录本的神经元数目（16.9 ± 1.0个/弓状切片）显著高于其他各组。动情前期（8.9 ± 1.8个）和动情间期（4.8 ± 1.0个）大鼠弓状核节段NKB神经元数目也有显著性差异。在注射戊酸雌二醇的大鼠中检测到最低数量的神经元（2.9 ± 0.6个NKB神经元/弓状切片）。此外，放射自显影颗粒密度的NKB神经元的卵巢切除组相比，所有其他组的两倍。与此相反，少数SP神经元被确定在大鼠弓状核和没有变化，在发情周期或卵巢切除术。而戊酸雌二醇组SP神经元的平均细胞面积减少。在POMC弓状神经元中检测到的唯一变化是戊酸雌二醇大鼠的细胞数量减少。这些数据表明，大鼠弓状核中的NKB神经元受到卵巢激素的调节，并支持绝经后妇女下丘脑中NKB基因表达增加是由于卵巢功能衰竭的假设。
Hypertrophy and increased gene expression of tachykinin neurons occur in the infundibular (arcuate) nucleus of postmenopausal women. We have hypothesized that the alterations in tachykinin gene expression in the hypothalami of postmenopausal women are secondary to ovarian failure and not due to age per se. In this study, in situ hybridization and computer-assisted microscopy were used to determine whether ovariectomy modulates neurokinin B (NKB), substance P (SP) or proopiomelanocortin (POMC) gene expression in the rat arcuate nucleus. Four groups were examined: proestrus; diestrous day 1; ovariectomized, and constant estrus induced by a single injection of 20 mg/kg estradiol valerate. Rats were sacrificed 2 months after treatment. Computer-assisted microscopy was used to determine the number of tachykinin neurons, cell areas, and the autoradiographic grain density of labeled neurons. We report marked changes in NKB gene expression in ovariectomized rats. The number of neurons containing NKB gene transcripts was significantly greater in ovariectomized rats (16.9 ± 1.0 neurons/arcuate section) than all other groups. There was also a significant difference in the number of NKB neurons/arcuate section between proestrous (8.9 ± 1.8 neurons) and diestrous (4.8 ± 1.0 neurons) rats. The lowest number of neurons was detected in the estradiol valerate-injected rats (2.9 ± 0.6 NKB neurons/ arcuate section). Furthermore, the autoradiographic grain density of NKB neurons was doubled in the ovariectomized group compared to all other groups. In contrast, few SP neurons were identified in the rat arcuate nucleus and no changes were detected during the estrous cycle or in response to ovariectomy. However, the mean cell area of SP neurons was decreased in the estradiol valerate rats. The only change detected in POMC arcuate neurons was a decreased number of cells in the estradiol valerate rat. These data indicate that NKB neurons in the rat arcuate nucleus are modulated by ovarian hormones and support the hypothesis that increased NKB gene expression in the hypothalamus of postmenopausal women is due to ovarian failure.