Correlation between circulating innate lymphoid cell precursors and thymic function.
Correlation between circulating innate lymphoid cell precursors and thymic function.
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DOI：
10.1016/j.isci.2022.103732
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发表时间：
2022-02-18
期刊：
影响因子：
5.8
通讯作者：
Sun XH
中科院分区：
文献类型：
--
作者：
Bajana S;Pankow A;Liu K;Michniowska M;Urban JF Jr;Chen WR;Sun XH
胸腺具有很高的支持ILCs分化的能力，尤其是当E蛋白转录因子被去除时。目前尚不清楚它是否有助于组织中ILC池的动态平衡。单细胞RNA测序分析表明，野生型裸血中有大量的ILC前体，但无瘤裸血中没有。CD3前体在细胞内(Ic)表达，但不在表面表达。LIN−CD127+CD62L+ICCD3ε+前体细胞丰度随年龄变化，在2-3个月龄达到高峰。这些细胞在体外可在OP9-DL1基质上分化为不同的ILC亚群。在肺、小肠和表皮中，ICCD3ε+细胞在稳定状态下在不同的组织环境中分化为不同的ILC亚群。蠕虫感染促进它们向功能性ILC2分化。因此，胸腺似乎在补充不同外周组织中的ILC池方面发挥了作用。由于胸腺活动与年龄有关，这一发现可能有助于解释与年龄相关的免疫反应差异。单细胞核酸测序检测血液中胸腺依赖(TD)的ILC前体细胞内(IC)而不是表面CD3ILCε标记血液和组织中的TD-ILC体外分化为不同的ILC亚群体外蠕虫感染促进ICCD3ILC+ILC2s的成熟免疫学；免疫系统的组成部分；OMICS；转录组学；ε；
The thymus has a high capacity to support the differentiation of ILCs, especially when E protein transcription factors are ablated. Whether it contributes to the homeostasis of ILC pools in tissues is not clear. Single-cell RNA sequencing analysis shows a substantial amount of ILC precursors in wild type but not athymic nude blood. The precursors express CD3 intracellularly (ic) but not on the surface. The abundance of Lin−CD127+CD62L+icCD3ε+ precursors varies with age, peaking at 2–3 months. These cells can differentiate into various ILC subsets on OP9-DL1 stroma in vitro. In the lung, small intestine, and epidermis, icCD3ε+ cells differentiate into diverse ILC subsets in different tissue environments in steady state. Helminth infection promotes their differentiation toward functional ILC2s. Thus, the thymus appears to play a role in replenishing ILC pools in different peripheral tissues. Because thymic activity is age-dependent, this finding may help explain age-related differences in immune responses. Single-cell RNA sequencing detects thymus-dependent (td) ILC precursors in the blood Intracellular (ic) but not surface CD3ε marks td-ILCs in the blood and tissues Blood td-ILCs differentiate into distinct ILC subsets in vitro Helminth infection promotes the maturation of icCD3ε+ ILC2s Immunology; Components of the immune system; Omics; Transcriptomics;
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DOI：
10.1016/j.jaci.2016.08.034
发表时间：
2017-05
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
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DOI：
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发表时间：
2010-07-02
期刊：
Science (New York, N.Y.)
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影响因子：
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作者：
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影响因子：
32.4
作者：
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影响因子：
30.5
作者：
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