Monitoring the Stimulated Release of Dopamine with In Vivo Voltammetry. I: Characterization of the Response Observed in the Caudate Nucleus of the Rat
Monitoring the Stimulated Release of Dopamine with In Vivo Voltammetry. I: Characterization of the Response Observed in the Caudate Nucleus of the Rat
复制标题
用体内伏安法监测多巴胺的刺激释放。
DOI：
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复制
发表时间：
1984
影响因子：
4.7
通讯作者：
R. Wightman
中科院分区：
文献类型：
--
作者：
W. Kuhr;A. Ewing;W. Caudill;R. Wightman
摘要：在麻醉大鼠脑内采用微伏安电极监测电刺激内侧前脑束后细胞外液中的化学物质。在尾状核和延髓核中观察到易氧化物质的浓度增加。大量证据表明，在刺激后观察到的物质是多巴胺。(1)刺激电极的位置必须在已知的多巴胺能神经束中以诱导释放。(2)释放最容易在含有大量含多巴胺神经元的大脑区域中观察到。(3)具有非常不同的电化学响应的两个伏安电极提供所释放的物种的伏安图，所述物种在一种情况下对于儿茶酚是独特的，在另一种情况下对于儿茶酚胺是独特的。(4)通过死后分析在纹状体组织中发现的3，4-二羟基苯乙酸的量与释放的多巴胺的计算量相关。(5)抑制酪氨酸羟化酶，从而多巴胺的合成，减少所观察到的释放，而抑制单胺氧化酶，从而形成多巴胺代谢物，不（6）的依赖性释放刺激参数与灌注技术获得的结果一致。因此，已经开发出一种新的方法来表征内源性多巴胺在大鼠脑中的释放，并可用于秒的时间尺度。
Abstract: Microvoltammetric electrodes were employed in the brain of an anesthetized rat to monitor chemical substances in extracellular fluid following electrical stimulation of the medial forebrain bundle. An increase in concentration of an easily oxidized substance is observed in the caudate nucleus and in the nucleus accumbens. A large amount of evidence suggests that the substance that is observed following stimulation is dopamine. (1) The location of the stimulating electrode must be in known dopaminergic tracts to induce release. (2) Release is most easily observed in brain regions that contain significant numbers of dopamine‐containing neurons. (3) Two voltammetric electrodes with very different electrochemical responses provide voltammograms of the released species that are unique for catechols in one case and catecholamines in another case. (4) The amount of 3,4‐dihydroxyphenylacetic acid found in striatal tissue by postmortem analysis correlates with the calculated amount of dopamine released. (5) Inhibition of tyrosine hydroxylase, and thus dopamine synthesis, decreases the observed release while inhibition of monoamine oxidase, and thus formation of dopamine metabolites, does not (6) The dependence of release on stimulation parameters agrees with results obtained with perfusion techniques. Thus, a new method has been developed to characterize endogenous dopamine release in the rat brain and can be used on a time scale of seconds.
影响因子：
5.5
作者：
NICHOLSON, C;PHILLIPS, JM
通讯作者：
PHILLIPS, JM
麻醉剂和电刺激对黑质纹状体多巴胺能神经元的影响。
DOI：
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发表时间：
1983
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
McCown,TJ;Mueller,RA;Breese,GR
通讯作者：
Breese,GR