Fluorescence-, isotope- or biotin-labeling of the 5'-end of single-stranded DNA/RNA using T4 RNA ligase
Fluorescence-, isotope- or biotin-labeling of the 5'-end of single-stranded DNA/RNA using T4 RNA ligase
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DOI：
10.1093/nar/25.18.3747
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发表时间：
1997-09-15
影响因子：
14.9
通讯作者：
Husimi, Y
中科院分区：
文献类型：
--
作者：
Kinoshita, Y;Nishigaki, K;Husimi, Y
开发了一种单链 DNA/RNA 的快速 5' 标记方法，该方法基于在 T4 RNA 连接酶反应中利用腺苷酸化中间体。该方法通常可用于寡核苷酸和多核苷酸 5' 端的荧光、同位素或生物素标记。
A rapid 5'-labeling method of single-stranded DNA/RNA was developed, which is based on the utilization of an adenylated intermediate in the reaction of T4 RNA ligase. This method is commonly useful for fluorescence-, isotope- or biotin-labeling of the 5'-ends of both oligo- and polynucleotides.