Cellular Localization of Transforming Growth Factor-f3 Expression In Bleomycin-Induced Pulmonary Fibrosis
Cellular Localization of Transforming Growth Factor-f3 Expression In Bleomycin-Induced Pulmonary Fibrosis
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DOI：
10.1097/00000441-193202000-00001
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Kai Zhang;K. Flanders;S. Phan
中科院分区：
文献类型：
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作者：
Kai Zhang;K. Flanders;S. Phan
博莱霉素诱导的肺纤维化与肺转化生长因子- 1 （TGF- 3）基因表达增加有关，但这种表达来源的细胞定位尚未明确。本研究在第0天气管内注射博来霉素诱导大鼠肺纤维化，并在选择的时间内采集肺进行原位杂交、免疫组化和组织化学分析TGF- 0.31 mRNA和蛋白表达，以及细胞细胞鉴定。结果显示，对照组肺实质中基本不表达可检测到的TGF-13、mRNA或蛋白。博来霉素治疗后第3天，分散的细支气管上皮细胞、单核细胞和嗜酸性粒细胞表达TGF-f31水平升高。在第3天至第14天，嗜酸性粒细胞、肌成纤维细胞和强表达TGF-f31 mRNA和蛋白的成纤维细胞的数量显著增加。产生TGF- 13的细胞主要局限于损伤区域和活动性纤维化。第14天后，表达tgf -f61的ceu的强度和数量显著下降，主要出现在纤维化区域的成纤维细胞中。除细支气管上皮细胞外，TGF- 11蛋白的表达与mRNA的表达基本一致
Bleomycin-induced pulmonary fibrosis is associated with increased lung transforming growth factor-"l (TGF-(3) gene expression, but celular localization of the source of this expression has not been unequivocally established. In this study, lungfibrosis was induced in rats by endotracheal bleomycin injection on day 0 and, on selected days afterwards, lungs were harvested for in situ hybridization, immunohistochemical and histochemical analyses for TGF-.31 mRNA and protein expression, and ceUl identification. The results show that control lungs express essentially no de-tectable TGF-13, mRNA or protein in the parenchyma. Before day 3 after bleomycin treatment, scattered bronchiolar epithelial cells, mononuclear cells, and eosinophils expressed elevated levels of TGF-f31. Between days 3 and 14, there was a major increase in the number of eosinophils, myofibroblasts, andfibroblasts strongly expressing TGF-f31 mRNA and protein. TGF- 13 -producing cells were predominantly localized within areas of injury and active fibrosis. After day 14, the intensity andnumber of TGF-f61-expressing ceUs significantly declined and were predominantlyfound infibroblasts in fibrotic areas. The expression of TGF-,l1protein was generally coincident with that for mRNA with the exception of bronchiolar epithelial cells in which strong protein expression