Bcmfs1, a novel major facilitator superfamily transporter from Botrytis cinerea, provides tolerance towards the natural toxic compounds camptothecin and cercosporin and towards fungicides
Bcmfs1, a novel major facilitator superfamily transporter from Botrytis cinerea, provides tolerance towards the natural toxic compounds camptothecin and cercosporin and towards fungicides
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DOI：
10.1128/aem.68.10.4996-5004.2002
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发表时间：
2002-10-01
影响因子：
4.4
通讯作者：
De Waard, MA
中科院分区：
文献类型：
--
作者：
Hayashi, K;Schoonbeek, HJ;De Waard, MA
克隆了灰葡萄孢菌（Botrytis cinerea）中一个新的主要易化因子超家族基因Bcmfs1，并构建了其置换突变体和过表达突变体，以研究其功能。替换突变体表现出增加的敏感性，天然有毒化合物喜树碱和尾孢菌素，分别由植物喜树和植物病原真菌尾孢菊池，产生。过表达突变体表现出对这些化合物和结构无关的杀真菌剂，如甾醇脱甲基化抑制剂（DMIs）的敏感性降低。Bcmfs1和ATP结合盒（ABC）转运蛋白基因BcatrD的双置换突变体比已知编码DMIs的重要ABC转运蛋白的BcatrD的单置换突变体对杀菌剂更敏感。野生型菌株和突变体的敏感性与BCMFS1的表达水平，并与这些菌株的幼苗的初始积累的oxpoconazole杀菌剂。结果表明，Bcmfs1是一个主要的促进剂超家族多药物转运蛋白参与保护对天然毒素和杀真菌剂，并具有底物特异性，与ABC转运蛋白BcatrD重叠。Bcmfs1可能参与了对B的保护作用。灰霉病菌在寄主植物上生长的致病阶段期间抵抗植物防御化合物，并且在其生长的杀菌阶段期间抵抗真菌毒性抗微生物代谢物。
Bcmfs1, a novel major facilitator superfamily gene from Botrytis cinerea, was cloned, and replacement and overexpression mutants were constructed to study its function. Replacement mutants showed increased sensitivity to the natural toxic compounds camptothecin and cercosporin, produced by the plant Camptotheca acuminata and the plant pathogenic fungus Cercospora kikuchii, respectively. Overexpression mutants displayed decreased sensitivity to these compounds and to structurally unrelated fungicides, such as sterol demethylation inhibitors (DMIs). A double-replacement mutant of Bcmfs1 and the ATP-binding cassette (ABC) transporter gene BcatrD was more sensitive to DMI fungicides than a single-replacement mutant of BcatrD, known to encode an important ABC transporter of DMIs. The sensitivity of the wild-type strain and mutants to DMI fungicides correlated with Bcmfs1 expression levels and with the initial accumulation of oxpoconazole by germlings of these isolates. The results indicate that Bcmfs1 is a major facilitator superfamily multidrug transporter involved in protection against natural toxins and fungicides and has a substrate specificity that overlaps with the ABC transporter BcatrD. Bcmfs1 may be involved in protection of B. cinerea against plant defense compounds during the pathogenic phase of growth on host plants and against fungitoxic antimicrobial metabolites during its saprophytic phase of growth.